Role of Ku antigen in the repair of apurinic/apyrimidinic sites in DNA
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Apurinic/apyrimidinic (AP) sites are some of the most frequent lesions in genomic DNA. It is widely accepted that, regardless of their origin, AP sites are further processed by base excision repair (BER) machinery, being the central intermediate of this process. Proteins that recognize AP sites are able to form covalent adducts with DNA under special conditions. Using a combination of the crosslinking technique with mass-spectrometry analysis, Ku antigen (Ku) (the central player in nonhomologous end joining (NHEJ), which is the pathway of double-strand break (DSB) repair), was identified as a protein reactive to AP sites. Moreover, Ku was shown to be 5′-dRP/AP lyase, which acts near DSBs in NHEJ. Recent studies have demonstrated the involvement of Ku in the different stages of BER. Here, Ku functions in the NHEJ and BER pathways of DNA repair were overviewed.
KeywordsKu antigen apurinic/apyrimidinic site nonhomologous end joining base excision repair
- AP site
apurinic/apyrimidinic endonuclease 1
DNA polymerase β
base excision repair
nonhomologous end joining
DNA-dependent protein kinase
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- 11.Levina E.S., Bavykin S.G., Shik V.V., Mirzabekov A.D. 1980. Interaction of histones with DNA in chromatin. A new method of covalent binding of histones to DNA available for their localization on DNA. Biochemistry (Moscow). 45, 1133–1145.Google Scholar
- 31.Choi Y.J., Li H., Son M.Y., Wang X.H., Fornsaglio J.L., Sobol R.W., Lee M., Vijg J., Imholz S., Dollé M.E., van Steeg H., Reiling E., Hasty P. 2014. Deletion of individual Ku subunits in mice causes an NHEJ-independent phenotype potentially by altering apurinic/apyrimidinic site repair. PLoS ONE. 9, e86358.CrossRefPubMedCentralPubMedGoogle Scholar
- 32.Ju Y.J., Lee K.H., Park J.E., Yi Y.S., Yun M.Y., Ham Y.H., Kim T.J., Choi H.M., Han G.J., Lee J.H., Lee J., Han J.S., Lee K.M., Park G.H. 2006. Decreased expression of DNA repair proteins Ku70 and Mre11 is associated with aging and may contribute to the cellular senescence. Exp. Mol. Med. 38, 686–693.CrossRefPubMedGoogle Scholar