Feedback between fluidity of membranes and transcription of the desB gene for the ω3-desaturase in the cyanobacterium Synechocystis
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Cells of prokaryotes (including cyanobacteria) respond to a decrease in the environmental temperature by activation of multiple genes with a low-temperature response. A decrease in temperature causes a reduction of the cell membrane fluidity, which is maintained at an optimum level due to the activity of fatty acid (FA) desaturases. We studied a temperature-dependent expression of the desB gene for the ω3-desaturase in the cyanobacterium Synechocystis, which is able to synthesize polyunsaturated FA, as well as in its double mutant (desA −/desD −), defective in the Δ12- and Δ6-desaturase genes, for which the presence of only monounsaturated FA is typical. During a decrease in temperature, in wild type cells the amount of desB mRNA increased, reaching a maximum value at 24°C. In the desA −/desD − double mutant, an accumulation of the desB transcript was characterized by a maximum at 28–30°C. Thus, using the desB gene encoding the ω3-FA-desaturase, it was demonstrated that a temperature-dependent expression of genes responsible for the maintenance of an optimal fluidity of cell membranes is determined by a physical state of these membranes and is regulated by a feedback mode.
KeywordsSynechocystis fluidity of membranes cold stress expression of genes
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- 4.Los D.A., Zinchenko V.V. 2009. Regulatory role of membrane fluidity in gene expression. In: Lipids in Photosynthesis. Eds. Wada H., Murata N. Springer, pp. 329–348.Google Scholar
- 5.Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno Ai, Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. 1996. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803: 2. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3, 109–136.PubMedCrossRefGoogle Scholar
- 8.Vigh L., Los D.A., Horvath I., Murata N. 1993. The primary signal in the biological perception of temperature: Pd-catalyzed hydrogenation of membrane lipids stimulates the expression of the desA gene in Synechocystis PCC6803. Proc. Natl. Acad. Sci. U. S. A. 90, 9090–9094.PubMedCrossRefGoogle Scholar
- 15.Rippka R., Deruelles J., Waterbury J., Herdman M., Stanier R. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Gen. Microbiol. 111, 1–61.Google Scholar
- 16.Grigorieva G., Shestakov S. 1982. Transformation in the cyanobacterium Synechocystis sp. PCC 6803. FEMS Microbiol. Lett. 13, 367–370.Google Scholar
- 19.Sambrook J., Fritsch E.F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press.Google Scholar