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Molecular Biology

, Volume 45, Issue 6, pp 950–958 | Cite as

Modulation of activated oncogene c-kit expression with RNA-interference

  • P. V. Spirin
  • N. A. Nikitenko
  • T. D. Lebedev
  • P. M. Rubtsov
  • C. Stocking
  • V. S. PrasolovEmail author
Molecular Biology of the Cell

Abstract

Overexpression of oncogene c-kit is detected in 80% patients with acute myeloid leukemia (AML). A transgenic model cell line expressing oncogene c-kit was obtained by transduction with a recombinant retrovirus. We have designed small interfering RNAs (siRNAs) that efficiently suppress the expression of activated oncogene c-kit. Further, small hairpin RNAs (shRNAs) targeting c-kit mRNA were designed and expressed in lentiviral vectors. We report a stable reduction in c-kit expression following the introduction of shRNAs into model cells, as well as Kasumi-1 cells from a patient with AML.

Keywords

RNA-interference small interfering RNA (siRNA) small hairpin RNA (shRNA) lentiviral vectors regulation of gene expression posttranscriptional regulation of expression acute myeloid leukemia (AML) 

Abbreviations

AML

acute myeloid leukemia

HSC

hemopoietic stem cell

FAB

French-American-British classification system of leukemias

siRNA

small interfering RNA

shRNA

small hairpin RNA

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • P. V. Spirin
    • 1
  • N. A. Nikitenko
    • 1
  • T. D. Lebedev
    • 1
  • P. M. Rubtsov
    • 1
  • C. Stocking
    • 2
  • V. S. Prasolov
    • 1
    Email author
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Heinrich-Pette-Institute for Experimental Virology and ImmunologyHamburgGermany

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