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Molecular Biology

, Volume 43, Issue 4, pp 552–556 | Cite as

Analysis of the 5′-leader regions of several plastid genes in protozoa of the phylum apicomplexa and red algae

  • T. A. Sadovskaya
  • A. V. Seliverstov
Genomics. Transcriptomics. Proteomics

Abstract

Apicomplexan parasites contain so-called apicoplasts, which are similar to chloroplasts of red algae. Multiple alignments of the 5′-leader regions of plastid-encoded genes revealed several conserved noncoding regions in parasites as well as in red algae. The regions were assumed to be sites for RNA interactions with regulatory proteins. Conserved sites were found upstream of ycf24, which is required for [Fe-S] cluster development, and several other genes. In particular, a simultaneous regulation was predicted for ycf24, rps4, and rpo B in Toxoplasma gondii. The prediction agreed with the known data that apicoplasts are only required for a short time, but confer pathogenicity on T. gondii. Another site was predicted upstream of rpo B, which encodes the β subunit of RNA polymerase, in red algae Porphyra spp. and parasites Eimeria tenella and Theileria parva.

Key words

gene regulation ycf24 rpoplastids comparative genomics Apicomplexa algae 

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Skryabin State Academy of Veterinary Medicine and BiotechnologyMoscowRussia
  2. 2.Kharkevich Institute of Information Transmission ProblemsRussian Academy of SciencesMoscowRussia

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