Exon-intron structure of the LY6G6D gene
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Loss of heterozygosity at microsatellite marker D6S273 within the HLA class III region was earlier observed at a high frequency in DNA samples from cervical intraepithelial neoplasias and cervical cancers. Three genes were identified in the region according to published data. The mRNA sequences available from GenBank were examined in silico, the exon-intron structure of the genes was analyzed, and the LY6G6D expression was studied in tumor cell lines. LY6G6D proved to consist of nine exons and to include, earlier identified, G6D and G6F. D6S273 was localized to the last intron 8 of LY6G6D. The third gene, LY6G6E, consists of four exons and is in intron 6 of LY6G6D in the opposite orientation. LY6G6D was assumed to have three main mRNA transcripts, which are encoded by the same open reading frame but differ in exon composition: MEGT1 consists of exons 1–4, 8, and 9; G6F consists of exons 1–6; and G6D consists of exons 7–9 of LY6G6D. A high homology to the immunoglobulin superfamily was observed within region 20–120 of the MEGT1 and G6F proteins by in silico translation of their mRNAs.
Key wordsLY6G6D in silico exon-intron structure D6S273
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- 3.Chatterjee A., Pulido H.A., Koul S., Beleno N., Perilla A., Posso H., Manusukhani M., Murty V.V. 2001. Mapping the sites of putative tumor suppressor genes at 6p25 and 6p21.3 in cervical carcinoma: occurrence of allelic deletions in precancerous lesions. Cancer Res. 61, 2119–2123.PubMedGoogle Scholar
- 4.Mazurenko N.N., Bliyev A.Yu., Bidzhieva B.A., Peskov D.Yu., Snigur N.V., Savinova E.B., Kisseliov F.L. 2006. Loss of heterozygosity at chromosome 6 as a marker of early genetic alterations in cervical intraepithelial neoplasias and microinvasive carcinomas. Mol. Biol. 40, 1–125.CrossRefGoogle Scholar
- 5.Mazurenko N., Beliakov I., Bliyev A., Guo Z., Hu X., Vinokourova S., Pavlova L., Ponten J., Kisseljov F. 2003. Genetic alterations at chromosome 6 associated with cervical cancer progression. Mol. Biol. 38, 472–481.Google Scholar
- 7.Ribas G., Neville M., Wixon J.L., Cheng J., Campbell R.D. 1999. Genes encoding three new members of the leukocyte antigen 6 superfamily and a novel member of Ig superfamily, together with genes encoding the regulatory nuclear chloride ion channel protein (hRNCC) and an N omega-N omega-dimethylarginine dimethylaminohydrolase homologue, are found in a 30-kb segment of the MHC class III region. J. Immunol. 163, 278–287.PubMedGoogle Scholar
- 22.Pascual M., Mataran L., Jones G., Shing D., van der Slik A.R., Giphart M.J., Schreuder G.M., de Vries R.R., Breedveld F.C., Roovers E., Zanelli E., Martin J. 2002. HLA haplotypes and susceptibility to rheumatoid arthritis: More than class II genes. Scand. J. Rheumatol. 31, 275–278.PubMedCrossRefGoogle Scholar