Molecular Biology

, Volume 40, Issue 2, pp 312–323 | Cite as

Mapping of antigenic determinants of hepatitis C virus proteins using phage display

  • E. A. Rechkina
  • G. F. Denisova
  • O. V. Masalova
  • L. F. Lideman
  • D. A. Denisov
  • E. I. Lesnova
  • R. I. Ataullakhanov
  • S. V. Gurianova
  • A. A. Kushch


Analysis of the properties for individual hepatitis C virus (HCV) proteins makes it possible to establish their molecular structure and conformation, to localize antigenic and immunogenic determinants, to identify protective epitopes, and to solve applied problems (e.g., design of diagnostic tests, vaccines, and drugs). Linear and conformational epitopes of HCV proteins were localized using the phage display technique, and the peptides exposed on the phages selected with monoclonal antibodies against HCV proteins were tested for immunogenicity. Of the 11 epitopes revealed, three were strongly linear; two depended on the secondary; and one on the tertiary structure of the corresponding protein (conformational epitopes). Amino acid sequences involved in the other epitopes were established. The results can be used to improve the diagnosis of hepatitis C, to study the effect of amino acid substitutions on the antigenic properties of HCV proteins, and to analyze the immune response in patients infected with genotypically different HCV. It was shown with the example of the NS5A epitope that phage particles with epitope-mimicking peptides (mimotopes) induce production of antibodies against the corresponding HCV proteins.

Key words

hepatitis C virus proteins antigenic determinants epitope mapping phage display immunogenic properties 


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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • E. A. Rechkina
    • 1
  • G. F. Denisova
    • 2
  • O. V. Masalova
    • 1
  • L. F. Lideman
    • 1
  • D. A. Denisov
    • 2
  • E. I. Lesnova
    • 1
  • R. I. Ataullakhanov
    • 3
  • S. V. Gurianova
    • 4
  • A. A. Kushch
    • 1
  1. 1.Ivanovsky Institute of VirologyRussian Academy of Medical SciencesMoscowRussia
  2. 2.Department of Cell Research and Immunology, Wise Faculty of Life SciencesTel-Aviv UniversityTel-AvivIsrael
  3. 3.Immapharma Joint-Stock CompanyMoscowRussia
  4. 4.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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