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Cell Ultrastructure in Azospirillum brasilense Biofilms

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Abstract

Due to the primary localization of both epiphytic and endophytic plant growth-promoting rhizobacteria on the surface of the plant root system, biofilm formation is an adaptive trait for these microorganisms. Under conditions of nitrogen limitation in liquid media, nitrogen-fixing Azospirillum brasilense strains switch mainly to the biofilm mode of growth. Overall ultrastructural similarities of the cells within A. brasilense biofilms were revealed, and their resistance to desiccation and oxidative stress was characterized. In strains Sp7, Cd, and Sp245, several types of single and undivided cells were revealed, as well as cystlike cells with pronounced morphological diversity. Resistance to desiccation and to oxidative stress was higher in the biofilm populations of these strains than in planktonic cultures. Dormant forms remained viable in dry biofilms of strains Sp245, Sp7, and Cd formed in a nitrogen-free medium after storage for 120 days. Viability of the biofilms of the same strains formed in the presence of nitrogen was retained for 120, 90, and 60 days, respectively. The minimal inhibitory concentration of H2O2 for biofilms was 1.0% for strains Sp7 and Cd and 0.1% for strain Sp245. Both the dormant forms and biofilms of strain Sp245 were more sensitive to H2O2 than those of strains Sp7 and Cd. Peroxidase activity was not previously reported in Azospirillum biofilms.

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Funding

The study was partially supported by the Russian Foundation for Basic Research (project nos. 18-34-00089 and 17-08-01696).

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Correspondence to A. V. Shelud’ko or E. I. Katsy.

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The authors declare no conflict of interest. This article does not contain any studies involving animals or human participants performed by the authors.

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Translated by A. Panyushkina

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Shelud’ko, A.V., Mokeev, D.I., Evstigneeva, S.S. et al. Cell Ultrastructure in Azospirillum brasilense Biofilms. Microbiology 89, 50–63 (2020). https://doi.org/10.1134/S0026261720010142

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Keywords:

  • biofilms
  • ultrastructure
  • dormant forms
  • desiccation
  • oxidative stress