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Microbiology

, Volume 87, Issue 6, pp 777–782 | Cite as

Method for Isolation and Enumeration of Fungi Developing in Marine Sediments

  • E. N. Bubnova
  • M. L. Georgieva
  • O. A. Grum-Grzhimailo
EXPERIMENTAL ARTICLES
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Abstract

A new design for a small growth container which was used for investigation of the microbiota of the White Sea littoral and sublittoral sediments is presented. These chambers made it possible to observe development of fungal mycelium under conditions of a natural ecotope (marine sediments) and to isolate this mycelium as pure cultures. The mycobiota isolated from the containers was compared to that obtained by standard plating method of the same sediments. The mycelium developed in 10% of the containers installed. Intensity of growth was found to be lower in the sublittoral than in the littoral. Most fungi isolated from the containers did not produce spores in pure cultures. Apart from non-sporulating cultures, colonies of marine species Paradendryphiellasalina and Acremoniumfuci were obtained. Standard plating of the sediments often resulted in isolation of Penicillium and Tolypocladium species, while the share of sterile isolates was considerably lower than in the case of isolation using growth containers.

Keywords:

fungi marine fungi isolation of fungal cultures diversity White Sea 

Notes

ACKNOWLEDGMENTS

The work was supported by the Russian Foundation for Basic Research, projects 15-04-02722 (development of chamber design, field works at the littoral, and, partially, isolation of the cultures); 15-29-02533ofi_m (partially, isolation and identification of the cultures) and by the Russian Science Foundation, project no. 14-50-00029 (field work at the sublittoral and analysis of the results). Microscopy was carried out at the Microscopy Center, WSBS. The works at the sublittoral were carried out by the WSBS diving department.

REFERENCES

  1. 1.
    Andreakis, N., Høj, L., Kearns, P., Hall, M.R., Ericson, G., Cobb, R.E., Gordon, B.R., and Evans-Illidge, E., Diversity of marine-derived fungal cultures exposed by DNA barcodes: the algorithm matters, PLoS One, 2015, vol. 10, no. 8, e0136130.CrossRefGoogle Scholar
  2. 2.
    Bubnova, E.N. and Nikitin, D.A., Fungi in bottom sediments of the Barents and Kara seas, Rus. J. Mar. Biol., 2017, vol. 43, pp. 400–406.CrossRefGoogle Scholar
  3. 3.
    Carlile, M.J., Watkinson, S.C., and Gooday, G.W., The Fungi, 2nd ed., Academic, 2001.Google Scholar
  4. 4.
    Jeewon, R. and Hyde, K.D., Detection and diversity of fungi from environmental samples: traditional versus molecular approaches, in Advanced Techniques in Soil Microbiology, Varuma, A. and Oelmüller, R., Eds., Springer, 2007, ch. 1, pp. 1–15.Google Scholar
  5. 5.
    Gams, W., The analysis of communities of saprophytic microfungi with special reference to soil fungi, in Fungi in Vegetation Science, Winterhoff, W., Ed., Springer, 1992, ch. 6, pp. 183–223.Google Scholar
  6. 6.
    Kohlmeyer, J. and Kohlmeyer, E., Marine Mycology—The Higher Fungi, New York: Academic, 1979.Google Scholar
  7. 7.
    Nagahama, T. and Nagano, Y., Cultured and uncultured fungal diversity in deep sea environments, in Biology of Marine Fungi, Raghukumar, Ch., Ed., Springer, 2012, ch. 9, pp. 174–190.Google Scholar
  8. 8.
    Pivkin, M.V., Secondary marine fungi of the Sea of Japan and the Sea of Okhotsk, Extended Abstract Doctoral Dissertation, Moscow, 2010.Google Scholar
  9. 9.
    Pivkin, M.V., Kuznetsova, T.A., and Sova, V.V., Morskie griby i ikh metabolity (Marine Fungi and Their Metabolites), Vladivostok: Dal’nauka, 2006.Google Scholar
  10. 10.
    Rämä, T., Hassett, B.T., and Bubnova, E., Arctic marine fungi: from filaments and flagella to operation taxonomic units and beyond, Bot. Mar., 2017, vol. 60, pp. 433–452.CrossRefGoogle Scholar
  11. 11.
    Williams, S.T., Parkinson, D., and Burges, N.A., An examination of the soil washing technique by its application to several soils, Plant Soil, 1965, vol. 22, pp. 167–186.CrossRefGoogle Scholar
  12. 12.
    Zhang, T., Wang, N.F., Zhang, Y.Q., Liu, H.Y., and Yu, L.Y., Diversity and distribution of fungal communities in the marine sediments of Kongsfjorden, Svalbard (High Arctic), Sci. Rep., 2015, vol. 5, no. 14524, pp. 1–11.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • E. N. Bubnova
    • 1
  • M. L. Georgieva
    • 1
    • 2
  • O. A. Grum-Grzhimailo
    • 1
  1. 1.Lomonosov Moscow State UniversityMoscowRussia
  2. 2.Gause Institute of New AntibioticsMoscowRussia

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