Effect of long-term application of agrotechnical techniques and crops on soil microbial communities
Effects of long-term application of various fertilizers and crops on soil microbiomes in a long-term field experiment were investigated using the library of the 16S rRNA gene sequences obtained by highthroughput sequencing of the total DNA. The communities exhibited high diversity, with 655 microbial genera belonging to 34 phyla detected (31 bacterial and 3 archaeal ones). For analysis of the effect of the studied factors on community structure, a linear model was developed in order to simplify interpretation of the data of high-throughput sequencing and to obtain biologically important information. Liming was shown to modulate the effect of mineral fertilizers on the structure of microbial populations. The differences in the structure and alpha-diversity of microbial communities were shown to depend more on the crops and liming than on the fertilizers applied. Interaction between the crop factor and liming expressed as an ambiguous effect of liming on the microbiome in the presence of different plants was reliably demonstrated. Thus, in the case of barley and clover, liming resulted in increased taxonomic diversity of the community, while in the case of potato and flax it had an opposite effect.
Keywordsstationary experiment 16S rRNA high-throughput sequencing organic fertilizers mineral fertilizers microbiome molecular ecology soil liming
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- Caporaso, J.G., Kuczynski, J., Stombaugh, J., Bittinger, K., Bushman, F.D., Costello, E.K., Fierer, N., Pena, A.G., Goodrich, J.K., Gordon, J.I., Huttley, G.A., Kelley, S.T., Knights, D., Koenig, J.E., Ley, R.E., Lozupone, C.A., McDonald, D., Muegge, B.D., Pirrung, M., Reeder, J., Sevinsky, J.R., Turnbaugh, P.J., Walters, W.A., Widmann, J., Yatsunenko, T., Zaneveld, J., and Knight, R., QIIME allows analysis of high-throughput community sequencing data, Nature Methods, 2010, vol. 7, pp. 335–336.CrossRefPubMedPubMedCentralGoogle Scholar
- Dumova, V.A., Pershina, E.V., Merzlyakova, Ya.V., Kruglov, Yu.V., and Andronov, E.E., Main tendencies in formation of a soil microbial community under conditions of a stationary field experiment according to high-throughput sequencing of the 16S rRNA gene libraries, Sel’skokhozyaist. Biol., 2013, no. 5, pp. 85–92.Google Scholar
- Pershina, E.V., Tamazyan, G.S., Dol’nik, A.S., Pinaev, A.G., Sergaliev, N.H., and Andronov, E.E., Investigation of the structure of a microbial community of saline soils by high-throughput sequencing, Ekol. Genet., 2012, vol. 10, no. 2, pp. 31–38.Google Scholar
- Zul, D., Denzel, S., Kotz, A., and Overmann, J., Effects of plant biomass, plant diversity, and water content on bacterial communities in soil lysimeters: implications for the determinants of bacterial diversity, Appl. Environ. Microbiol., 2007, vol. 73, no. 21, pp. 6916–6929.CrossRefPubMedPubMedCentralGoogle Scholar