Methanocalculus natronophilus sp. nov., a new alkaliphilic hydrogenotrophic methanogenic archaeon from a soda lake, and proposal of the new family Methanocalculaceae
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A mesophilic hydrogenotrophic methanogenic archaeon, strain Z-7105T, was isolated from the bottom sediments of a collector in the vicinity of a soda lake Tanatar II (Altai, Russia). The cells were motile, irregular cocci 0.2–1.2 μm in diameter. The organism was an obligate alkaliphile, growing within a pH range from 8.0 to 10.2, with the optimum at pH 9.0–9.5. It was obligately dependent on carbonates, growing at 0.5 to 1.6 M total carbonates with the optimum at 0.7–0.9 M. Sodium ions were also obligately required at concentrations from 0.9 to 3.3 M Na+ (optimum at 1.4–1.9 M). The organism was halotolerant, but Clions were not required. Hydrogen and formate were used as electron donors. Acetate was required for anabolism. The DNA G+C content was 50.2 mol %. According to the results of its 16S rRNA gene sequence analysis, the isolate belonged to the genus Methanocalculus, being the first known alkaliphilic member of this genus. Its similarity to the neutrophilic and halotolerant Methanocalculus species (M. halotolerans, M. taiwanensis, M. pumilus, and M. chunghsingensis) was 98.2–97.1%, which is within the interspecific range for this genus. The level of DNA-DNA hybridization between strain Z-7105T and the Methanocalculus type species M. halotolerans DSM 14092T was 32%. The genus Methanocalculus, including the new isolate and the previously described species, is distant from other genera of methanogens (<90% 16S rRNA gene similarity). Based on significant phenotypic differences and the results of phylogenetic analysis, including DNA-DNA hybridization, it is proposed to assign strain Z-7105T (=DSM 25006T, =VKM B-2765T) to the new species Methanocalculus natronophilus sp. nov., and to incorporate the genus into the new family Methanocalculaceae fam. nov.
Keywordssoda lakes alkaliphiles methanogens hydrogenotrophs archaea Methanocalculus natronophilus Methanocalculaceae
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- 1.Zavarzin, G.A., Epicontinental soda lake are probable relict biotopes of terrestrial biota formation, Microbiology (Moscow), 1993, vol. 62, pp. 473–479.Google Scholar
- 3.Zavarzin, G.A., Zhilina, T.N., and Kevbrin, V.V., The alkaliphilic microbial community and its functional diversity, Microbiology (Moscow), 1999, vol. 68, pp. 503–521.Google Scholar
- 5.Trudy Instituta mikrobiologii im. S.N. Vinogradskogo (Proceedings of Winogradsky Institute of Microbiology), vol. 14: Alkalofil’nye mikrobnye soobshchestva (Alkaliphilic Microbial Communities), Gal’chenko, V.F., Ed., Moscow: Nauka, 2007.Google Scholar
- 6.Zavarzin, G.A., Evolyutsiya prokariotnoi biosfery: Mikroby v krugovorote zhizni: 120 let spustya: Chtenie im. S.N. Vinogradskogo (Evolution of the Prokaryotic Biosphere. Microbes in the Life Cycles: 120 Years Later: Lecture in Honor of S.N. Winogradsky), Kolotilova, N.N., Ed., Moscow: MAKS Press, 2011.Google Scholar
- 7.Zavarzina, D.G. and Zhilina, T.N., Anaerobic communities of soda lakes as analogues of Precambrian paleocontinental microbiota, Rannyaya kolonizatsiya sushi (Early Colonization of the Land), Rozhnov, S.V., Ed., Moscow: PIN RAN, 2012, pp. 69–91.Google Scholar
- 8.Zhilina, T.N., Zavarzina, D.G., Panteleeva, A.N., Osipov, G.A., Kostrikina, N.A., Tourova, T.P., and Zavarzin, G.A., Fuchsiella alkaliacetigena gen. nov., sp. nov., an alkaliphilic, lithoautotrophic homoacetogen from a soda lake, Int. J. Syst. Evol. Microbiol., 2012, vol. 62, pp. 1666–1673.PubMedCrossRefGoogle Scholar
- 9.Zavarzin, G.A., Zhilina, T.N., and Pikuta, E.V., Secondary anaerobes in haloalkaliphilic communities in lakes of Tuva, Microbiology (Moscow), 1996, vol. 65, no. 4, pp. 480–486.Google Scholar
- 10.Namsaraev, B.B., Zhilina, T.N., Kulyrova, A.V., and Gorlenko, V.M., Bacterial methanogenesis in soda lakes of the southeastern Transbaikal region, Microbiology (Moscow), 1999, vol. 68, pp. 586–591.Google Scholar
- 14.Kevbrin, V.V. and Zavarzin, G.A., The effect of sulfur compounds on the growth of halophilic homoacetic bacterium Acetohalobium arabaticum, Microbiology (Moscow), 1992, vol. 61, pp. 563–567.Google Scholar
- 17.Lane, D.J., 16S/23S rRNA sequencing, in Nucleic Acid Techniques in Bacterial Systematics, Stackebrandt, E. and Goodfellow, M., Eds., Chichester: John Wiley & Sons, 1991, pp. 115–175.Google Scholar
- 29.Sakai, S., Ehara, M., Tseng, I.-C., Yamaguchi, T., Brauer, S.L., Cadillo-Quiroz, H., Zinder, S.H., and Imachi, H., Methanolinea mesophila sp. nov., a hydrogenotrophic methanogen isolated from rice field soil, and proposal of the archaeal family Methanoregulaceae fam. nov. within the order Methanomicrobiales, Int. J. Syst. Evol. Microbiol., 2012, vol. 62, pp. 1389–1395.PubMedCrossRefGoogle Scholar