Effect of the reactivating factor of Luteococcus japonicus subsp. casei on the expression of SOS response genes
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The effect of the extracellular peptide reactivating factor (RF) synthesized by Luteococcus casei on stress response of Escherichia coli cells subjected to UV irradiation was studied. For these studies, we constructed a test strain carrying the umuD-lacZ operon. The expression rate of this operon reflects the rate of SOS response. Protective effect of RF, defined as the number of cells retaining the colony-forming activity (CFU) after UV irradiation (49–1166 J/m2), was dose-dependent, species-nonspecific, and increasing with increase of the stress load. RF was demonstrated to possess the properties of a direct adaptogen: 15 min of preincubation with RF caused a 1.5–6-fold decrease in expression of the umuD SOS response gene in UV-treated cells, concurrently with a 1.2–7.5 times increase in the number of viable cells (those having retained their colony-forming activity). The probable mechanisms of the protective effect of RF are being discussed.
Keywordsreactivating factor (RF) Luteococcus japonicus subsp. casei stress protection UV irradiation E. coli SOS response umuD-lacZ hybrid operon
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- 2.Basnak’yan, I.A., Stress u bakterii (Stress in Bacteria), Moscow: Meditsina, 2003.Google Scholar
- 8.Booth, I.R., Adaptation to Extreme Environments, in Biology of the Prokaryotes, Lengeler, J.W., Drews, G., and Schlegel, H.G., Eds., Stuttgart: Thieme, 1999 [Russ. Transl. Moscow: Mir, 2005, vol. 2, pp. 122–146].Google Scholar
- 11.Walker, C.G., The SOS Response in Escherichia coli, in Escherichia coli and Salmonella: Cellular and Molecular Biology, Neidhardt, F.C., Curtiss, R. III, Ingraham, J.L., Lin, E.C.C., Low, K.B. et al., Eds., Washington: ASM, 1996, pp. 1400–1416.Google Scholar
- 13.Rowbury, R.J., Cross-Talk Involving Extracellular Sensors and Extracellular Alarmones Gives Early Warning to Unstressed Escherichia coli of Impeding Lethal Chemical Stress and Leads to Induction of Tolerance Responses, J. Appl. Microbiol., 2001, vol. 90, no. 2, pp. 677–695.PubMedCrossRefGoogle Scholar
- 16.Golod, N.A., Loiko, N.G., Lobanov, K.V., Mironov, A.S., Voeikova, T.A., Gal’chenko, V.F., Nikolaev, Yu.A., and El’-Registan, G.I., Involvement of Alkylhydroxybenzenes, Microbial Autoregulators, in Controlling the Expression of Stress Regulons, Microbiology, 2009, vol. 78, no. 6, pp. 678–688.CrossRefGoogle Scholar
- 22.Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor Lab., 1972.Google Scholar