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Microbiology

, Volume 82, Issue 1, pp 91–101 | Cite as

Comparative analysis of biodiversity in the planktonic and biofilm bacterial communities in Lake Baikal

  • V. V. Parfenova
  • A. S. GladkikhEmail author
  • O. I. Belykh
Experimental Articles

Abstract

Bacterial communities of the water and the biofilm formed during five years on an artificial substrate in Lake Baikal were studied by the pyrosequencing of 16S rRNA gene fragments; taxonomic diversity of bacterial communities and differences in their structure were revealed. The biofilm community contained mainly representatives of three phyla: Cyanobacteria, Bacteroidetes, and Proteobacteria; the amounts of other groups were within 1%. Bacterial community of the plankton was more heterogeneous; along with the dominant phyla (Bacteroidetes, Actinobacteria, and Proteobacteria) 15% of the members were of the other phyla. The use of pyrosequencing allowed to reveal 35 bacterial phyla in Lake Baikal, some of which were identified for the first time; moreover, minor groups of microorganisms (including only several sequences), which were not earlier determined by other molecular methods were found.

Keywords

Lake Baikal pyrosequencing 16S rRNA plankton biofilm biodiversity Cyanobacteria Actinobacteria Bacteroidetes 

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Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • V. V. Parfenova
    • 1
  • A. S. Gladkikh
    • 1
    Email author
  • O. I. Belykh
    • 1
  1. 1.Limnological Institute, Siberian BranchRussian Academy of SciencesIrkutskRussia

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