Abstract
Phase variation in the culture of the environmental strain Lactococcus lactis subsp. lactis 194 resulted in the formation of two types of colonies differing by 15% in antibiotic activity. The active variant 194-K produced an antibiotic complex with a broad spectrum of antibacterial and antifungal activity. Five components (194-A, B, C, D, and E) were isolated from the complex by solid-phase extraction and thin-layer chromatography. Components 194-A and 194-B were hydrophobic neutral compounds soluble in organic solvents. Component 194-A possessed fungicidal activity, whereas component 194-B exhibited only bactericidal activity. Physicochemical studies of the isolated components 194-A and 194-B revealed that they had no analogs in the Berdy database of biologically active substances (BNPD) and appeared to be novel antibiotics. Component 194-C was a hydrophilic polar compound inhibiting growth of gram-positive and gramnegative bacteria. Component 194-D belonged to peptide antibiotics; it inhibited growth of only gram-positive bacteria and was similar to nisin A in biological properties but differed in electrophoretic mobility and molecular mass.
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Original Russian Text © E.A. Ustyugova, G.B. Fedorova, G.S. Katrukha, L.G. Stoyanova, 2011, published in Mikrobiologiya, 2011, Vol. 80, No. 5, pp. 644–650.
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Ustyugova, E.A., Fedorova, G.B., Katrukha, G.S. et al. Investigation of the antibiotic complex produced by Lactococcus lactis subsp. lactis 194, Variant K. Microbiology 80, 657–663 (2011). https://doi.org/10.1134/S002626171105016X
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DOI: https://doi.org/10.1134/S002626171105016X