Microbiology

, Volume 76, Issue 5, pp 557–562 | Cite as

The composition of the chitinolytic microbial complex and its effect on chitin decomposition at various humidity levels

  • A. V. Vorob’ev
  • N. A. Manucharova
  • A. M. Yaroslavtsev
  • E. V. Belova
  • D. G. Zvyagintsev
  • I. I. Sudnitsyn
Experimental Articles

Abstract

The dynamics of assimilation of chitin by soil microorganisms (primarily prokaryotes) as a source of carbon and nitrogen has been determined by gas chromatography and fluorescence microscopy. The highest rates of chitin decomposition in chernozem were detected at humidity levels corresponding to the pressure of soil moisture (P) of −1.4 atm. The rate of microbial consumption of chitin is three times higher than that of the carbon of soil organic matter. Fluorescence microscopy revealed that an increase in the pressure of soil moisture from P = −10 atm to P = −0.7 atm resulted in a considerable increase in the proportion of the specific surface of mycelial bacteria (actinomycetes).

Keywords

chitinolytic soil microorganisms chitinase complex mycelial actinobacteria soil moisture 

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References

  1. 1.
    Terekhov, A.S., Zenova, G.M., Kozhevin, P.A., and Mikhailova, N.V., Multisubstrate Testing of Soil Actinomycetes, Vestnik Moskovskogo Universiteta, Ser. 19, “Soil Science”, 2001, no. 2, pp. 10–13.Google Scholar
  2. 2.
    De Boer, W., Klein Gunnewiek, P.J.A., Lafeber, P., Janse, J.D., Spit, B.E., and Woldendorp, J.W., Antifungal Properties of Chitinolytic Dune Soil Bacteria, Soil Biol. Biochem., 1998, vol. 30, pp. 193–203.CrossRefGoogle Scholar
  3. 3.
    Chen, K.S., Lee, K.K., and Chen, H.C., A Rapid Method for Detection of N-acetglucosaminidase-Type Chitinase Activity in Crossed Immunoelectrophoresis and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Gels Using 4-methylumbelliferyl-N-acetyl-B-glucosaminide as Substrate, Electrophoresis, 1994, vol. 15, pp. 622–665.Google Scholar
  4. 4.
    Eltarabily, K.A., Soliman, M.H., Nassar, A.H., and Alhassani, H.A., Biologycal Control of Sclerotinia minor Using a Chitinolytic Bacterium and Actinomycetes, Plant Pathol., 2000, vol. 49,iss. 5, pp. 573–583.CrossRefGoogle Scholar
  5. 5.
    Sovremennaya mikrobiologiya (prokarioty) (Modern Microbiology (Prokaryotes)), Lengeler, I. et al., Moscow: Mir, 2005.Google Scholar
  6. 6.
    Aktuganov, G.E., Melent’ev, A.I., Kuz’mina, L.Yu., Galimzyanova, N.F., and Shirokov, A.V., The Chitinolytic Activity of Bacillus Cohn Bacteria Antagonistic to Phytopathogenic Fungi, Mikrobiologiya, 2003, vol. 72, no. 3, pp. 356–360 [Microbiology (Engl. Transl.), vol. 72, no. 3, pp. 313–317].Google Scholar
  7. 7.
    Gomez Ramirez, M., Rojas Avelizapa, L.I., Rojas Avelizapa, N.G., and Cruz Camarillo, R., Colloidal Chitin Stained with Remazol Brilliant Blue R, a Useful Substrate to Select Chitinolytic Microorganisms and to Evaluate Chitinases, J. Microbiol. Meth., 2004, vol. 56,iss. 2, pp. 213–219.CrossRefGoogle Scholar
  8. 8.
    Kalakutskii, L.V. and Agre, N.S., Razvitie aktinomitsetov (Development of Actinomycetes), Moscow: Nauka, 1977.Google Scholar
  9. 9.
    Hildgund Schrempf. Recognition and Degradation of Chitin by Streptomycetes, A. van Leeuwenhoek, 2001, vol. 79(3–4), pp. 285–289.CrossRefGoogle Scholar
  10. 10.
    Doroshenko, E.A., Zenova, G.M., Zvyagintsev, D.G., and Sudnitsyn, I.I., Spore Germination and Mycelial Growth of Streptomycetes at Different Humidity Levels, Mikrobiologiya, 2005, vol. 74, no. 6, pp. 795–799 [Microbiology (Engl. Transl.), vol. 74, no 6, pp. 690–694].Google Scholar
  11. 11.
    Folders, J., Algra, J., Roelofs, M.S., van Loon, L.C., Tommassen, J., and Bitter, W., Characterization of Pseudomonas aeruginosa Chitinase, a Gradually Secreted Protein, J. Bacteriol., 2001, vol. 183, no. 24, pp. 7044–7052.PubMedCrossRefGoogle Scholar
  12. 12.
    Patidar, P., Agrawal, D., Banerjee, T., and Patil, S., Optimization of Process Parameters for Chitinase Production by Soil Isolates of Penicillium chrysogenum under Solid Substrate Fermentation, Process Biochem., 2005, vol. 40,iss. 9, pp. 2962–2967.CrossRefGoogle Scholar
  13. 13.
    Dobrovol’skaya, T.G., Struktura bakterial’nykh soobshchestv pochv (Structure of Soil Bacterial Communities), Moscow: Akademkniga, 2002.Google Scholar
  14. 14.
    Zvyagintsev, D.G., Pochvy i mikroorganizmy (Soils and Microorganisms), Moscow: Mosk. Gos. Univ., 1987.Google Scholar
  15. 15.
    Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry), Moscow: Mosk. Gos. Univ., 1991, p. 303.Google Scholar
  16. 16.
    Kozhevin, P.A., Mikrobnye populyatsii v prirode (Microbial Populations in Nature), Moscow: Mosk. Gos. Univ., 1989.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  • A. V. Vorob’ev
    • 1
  • N. A. Manucharova
    • 1
  • A. M. Yaroslavtsev
    • 1
  • E. V. Belova
    • 1
  • D. G. Zvyagintsev
    • 1
  • I. I. Sudnitsyn
    • 1
  1. 1.Faculty of Soil ScienceMoscow State UniversityMoscowRussia

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