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Microbiology

, Volume 75, Issue 3, pp 329–335 | Cite as

Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation approaches

  • I. S. Kulichevskaya
  • T. A. Pankratov
  • S. N. DedyshEmail author
Experimental Articles

Abstract

By means of fluorescence in situ hybridization with 16S rRNA-targeted oligonucleotide probes (FISH), it has been shown that members of the phylum Planctomycetes represent a numerically significant bacterial group in boreal Sphagnum peat bogs. The population size of planctomycetes in oxic layers of the peat bog profile was in the range of 0.4–2.0 × 107 cells per g of wet peat, comprising 4 to 13% of the total bacterial cell number. A novel effective approach that combined a traditional cultivation technique with FISH-mediated monitoring of the target organism during the isolation procedure has been developed for the isolation of planctomycetes. Using this approach, we succeeded in isolating several peat-inhabiting planctomycetes in a pure culture. Sequencing of the 16S rRNA genes from two of these isolates, strains A10 and MPL7, showed that they belonged to the planctomycete lineages defined by the genera Gemmata and Planctomyces, respectively. The 16S rRNA gene sequence similarity between strains A10 and MPL7 and the phylogenetically closest organisms, namely, Gemmata obscuriglobus and Planctomyces limnophilus, was only 90%. These results suggest that the indigenous planctomycetes inhabiting Sphagnum peat bogs are so far unknown organisms.

Key words

the phylum Planctomycetes Sphagnum peat bogs fluorescence in situ hybridization Gemmata Planctomyces 

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Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • I. S. Kulichevskaya
    • 1
  • T. A. Pankratov
    • 1
  • S. N. Dedysh
    • 1
    Email author
  1. 1.Winogradsky Institute of MicrobiologyRussian Academy of SciencesMoscowRussia

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