, Volume 75, Issue 3, pp 329–335 | Cite as

Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation approaches

  • I. S. Kulichevskaya
  • T. A. Pankratov
  • S. N. DedyshEmail author
Experimental Articles


By means of fluorescence in situ hybridization with 16S rRNA-targeted oligonucleotide probes (FISH), it has been shown that members of the phylum Planctomycetes represent a numerically significant bacterial group in boreal Sphagnum peat bogs. The population size of planctomycetes in oxic layers of the peat bog profile was in the range of 0.4–2.0 × 107 cells per g of wet peat, comprising 4 to 13% of the total bacterial cell number. A novel effective approach that combined a traditional cultivation technique with FISH-mediated monitoring of the target organism during the isolation procedure has been developed for the isolation of planctomycetes. Using this approach, we succeeded in isolating several peat-inhabiting planctomycetes in a pure culture. Sequencing of the 16S rRNA genes from two of these isolates, strains A10 and MPL7, showed that they belonged to the planctomycete lineages defined by the genera Gemmata and Planctomyces, respectively. The 16S rRNA gene sequence similarity between strains A10 and MPL7 and the phylogenetically closest organisms, namely, Gemmata obscuriglobus and Planctomyces limnophilus, was only 90%. These results suggest that the indigenous planctomycetes inhabiting Sphagnum peat bogs are so far unknown organisms.

Key words

the phylum Planctomycetes Sphagnum peat bogs fluorescence in situ hybridization Gemmata Planctomyces 


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  1. 1.
    Fuerst, J.A., The Planctomycetes—Emerging Models for Microbial Ecology, Evolution and Cell Biology, Microbiology (UK), 1995, vol. 141, pp. 1493–1506.Google Scholar
  2. 2.
    Fuerst, J.A., Planctomycetes—a Phylum of Emerging Interest for Microbial Evolution and Ecology, WFCC Newsletter, 2004, vol. 38, pp. 1–11.Google Scholar
  3. 3.
    König, H., Schlesner, H., and Hirsch, P., Cell Wall Studies on Budding Bacteria of the Planctomyces/Pasteuria Group and on Prosthecomicrobium sp., Arch. Microbiol., 1984, vol. 138, pp. 200–205.CrossRefGoogle Scholar
  4. 4.
    Lindsay, M.R., Webb, R.I., Strous, M., Jetten, M.S., Butler, M.K., Forde, R.J., and Fuerst, J.A., Cell Compartmentalization in Planctomycetes: Novel Types of Structural Organization for the Bacterial Cell, Arch. Microbiol., 2001, vol. 175, pp. 413–429.PubMedCrossRefGoogle Scholar
  5. 5.
    Fuerst, J.A., and Webb, R.I., Membrane-Bounded Nucleoid in the Eubacterium Gemmata obscuriglobus, Proc. Natl. Acad. Sci. USA., 1991, vol. 88, pp. 8184–8188.PubMedGoogle Scholar
  6. 6.
    Glöckner, F.O., Kube, M., Bauer, M., Teeling, H., Lombardot, T., Ludwig, W., Gade, D., Beck, A., Borzym, K., Heitmann, K., Rabus, R., Schlesner, H., Amann, R., and Reinhardt, R., Complete Genome Sequence of the Marine Planctomycete Pirellula sp. strain 1, Proc. Natl. Acad. Sci. USA, 2003, vol. 100, pp. 8298–8303.PubMedCrossRefGoogle Scholar
  7. 7.
    Gimesi, N., Planctomyces bekefii Gim. nov. gen. et sp., Hydrobiologiai Tanulmányok, 1924, Budapest: Kiadja a Magyar Ciszterci Rend, pp. 1–8.Google Scholar
  8. 8.
    Krasil’nikov, N.A., Opredelitel’ bakterii i aktinomitsetov (Manual for Identification of Bacteria and Actinomycetes), Moscow-Leningrad: Akad. Nauk SSSR, 1949.Google Scholar
  9. 9.
    Staley, J.T., Budding Bacteria of the Pasteuria-Blastobacter Group, Can. J. Microbiol., 1973, vol. 19, pp. 609–614.PubMedCrossRefGoogle Scholar
  10. 10.
    Schlenser, H., The Development of Media Suitable for the Microorganisms Morphologically Resembling Planctomyces spp., Pirellula spp., and Other Planctomycetales from Various Aquatic Habitats Using Dilute Media, Syst. Appl. Microbiol., 1994, vol. 17, pp. 135–145.Google Scholar
  11. 11.
    Jetten, M.S.M., Cirpus, I., Kartal, B., van Niftrik, L., van de Pas-Schoonen, K.T., Sliekers, O., Haaijer, S., van der Star, W., Schmid, M., van de Vossenberg, J., Schmidt, I., Harhangi, H., van Loosdrecht, M., Kuene, J.G., Op den Camp, H., and Strous, M., 1994–2004: 10 Years of Research on the Anaerobic Oxidation of Ammonium, Biochem. Soc. Trans., 2005, vol. 33, pp. 119–123.PubMedCrossRefGoogle Scholar
  12. 12.
    Liesack, W. and Stackebrandt, E., Occurrence of Novel Groups of the Domain Bacteria as Revealed by Analysis of Genetic Material Isolated from an Australian Terrestrial Environment, J. Bacteriol., 1992, vol. 174, pp. 5072–5078.PubMedGoogle Scholar
  13. 13.
    Zarda, B., Hahn, D., Chatzinotas, A., Schonhuber, W., Neef, A., Amann, R.I., and Zeyer, J., Analysis of Bacterial Community Structure in Bulk Soil by In Situ Hybridization, Arch. Microbiol., 1997, vol. 168, pp. 185–192.CrossRefGoogle Scholar
  14. 14.
    Kuypers, M.M.M., Sliekers, A.O., Lavik, G., Schmid, M., Jørgensen, B.B., Kuenen, J.G., Damste, J.S.S., Strous, M., and Jetten, M.S.M., Anaerobic Ammonium Oxidation by Anammox Bacteria in the Black Sea, Nature, 2003, vol. 422, pp. 608–611.PubMedCrossRefGoogle Scholar
  15. 15.
    Strous, M., Fuerst, J.A., Kramer, E.H., Logemann, S., Muyzer, G., van de Pas-Schoonen, K.T., Webb, R., Kuenen, J.C., and Jetten, M.S., Missing Lithotroph Identified as New Planctomycete, Nature, 1999, vol. 400, pp. 446–449.PubMedCrossRefGoogle Scholar
  16. 16.
    Dedysh, S.N., Pankratov, T.A., Belova, S.E., Kulichevskaja, I.S., and Liesack, W., Phylogenetic Analysis and In Situ Identification of Bacterial Community Composition in an Acidic Sphagnum Peat Bog, Appl. Environ. Microbiol., 2005, vol. 72, pp. 2110–2117.CrossRefGoogle Scholar
  17. 17.
    Pankratov, T.A., Belova, S.E., and Dedysh, S.N., Evaluation of the Phylogenetic Diversity of Prokaryotic Microorganisms in Sphagnum Peat Bogs by Means of Fluorescence In Situ Hybridization (FISH), Mikrobiologiya, 2005, vol. 74, no. 6, pp. 831–837 [Microbiology [Engl. Transl.), vol. 74, no. 6, pp. 722–728].Google Scholar
  18. 18.
    Daims, H., Brühl, A., Amann, R., Schleifer, K.-H., and Wagner, M., The Domain-Specific Probe EUB338 is Insufficient for the Detection of all Bacteria: Development and Evaluation of a More Comprehensive Probe Set, Syst. Appl. Microbiol., 1999, vol. 22, pp. 434–444.PubMedGoogle Scholar
  19. 19.
    Neef, A., Amann, R., Schlesner, H., and Schleifer, K.-H., Monitoring a Widespread Bacterial Group: In Situ Detection of Planctomycetes with 16S rRNA-Targeted Probes, Microbiology (UK), 1998, vol. 144, pp. 3257–3266.Google Scholar
  20. 20.
    Hirsch, P., and Müller, M., Methods and Sources for the Enrichment and Isolation of Budding, Nonprosthecate Bacteria from Freshwater, Microb. Ecol., 1986, vol. 12, pp. 331–341.CrossRefGoogle Scholar
  21. 21.
    Staley, J.T., Fuerst, J.A., Giovannoni, S., and Schlesner, H., The Order Planctomycetales and the Genera Planctomyces, Pirellula, Gemmata and Isosphaera, The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, Balows, A., Trüper, H., Dworkin, M., Harder, W., and Schleifer, K.-H., Eds., New York: Springer-Verlag, 1992, pp. 3710–3731.Google Scholar
  22. 22.
    Dedysh, S.N., Panikov, N.S., and Tiedje, J.M., Acidophilic Methanotrophic Communities from Sphagnum Peat Bogs, Appl. Environ. Microbiol., 1998, vol. 64, no. 3, pp. 922–929.PubMedGoogle Scholar
  23. 23.
    Weisburg, W.G., Barns, S.M., Pelletier, D.A., and Lane, D.J., 16S Ribosomal DNA Amplification for Phylogenetic Study, J. Bacteriol., 1991, vol. 173, pp. 697–703.PubMedGoogle Scholar
  24. 24.
    Franzmann, P.D., and Skerman, V.D., Gemmata obscuriglobus, a New Genus and Species of the Budding Bacteria, Antonie van Leeuwenhoek, 1984, vol. 50, pp. 261–268.PubMedCrossRefGoogle Scholar
  25. 25.
    Hirsch, P., and Müller, M., Planctomyces limnophilus sp. nov., a Stalked and Budding Bacterium from Freshwater, Syst. Appl. Microbiol., 1985, vol. 6, pp. 276–280.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • I. S. Kulichevskaya
    • 1
  • T. A. Pankratov
    • 1
  • S. N. Dedysh
    • 1
    Email author
  1. 1.Winogradsky Institute of MicrobiologyRussian Academy of SciencesMoscowRussia

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