Fluorescence Spectral Study on the Interaction Between Copper(II) Complex with (E)-3-(2,3-Dihydrobenzo[b][1,4] Dioxin-6-Yl) Acrylic Acid and Urease
- 8 Downloads
We have previously reported the synthesis, structure and urease inhibitory activity of a complex [Cu2(L)4DMSO2]·2DMSO (HL = (E)-3-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylic acid), and we found the complex to show a strong inhibitory activity against jack bean urease . In this study, the interaction of the complex and urease is studied by fluorescence spectroscopy. We find that the fluorescence quenching mechanism of the complex with urease is static quenching. The quenching rate constant (Kq) is 1.6·1012 l/mol·s–1, the quenching constant (Ksv) is 1.6×104 l/mol, the association binding constant (K) is 3.55·104 l/mol, and the binding site number (n) is 1.22.
Keywordscomplex urease binding quenching mechanism Ksv Kq
Unable to display preview. Download preview PDF.
- 2.H. L. Mobley and R. P. Hausinger. Microbiol. Rev., 1989, 53(1), 85–108.Google Scholar
- 3.H. L. Mobley, M. D. Island, and R. P. Hausinger. Microbiol. Rev., 1995, 59(30), 451–480.Google Scholar
- 8.IARC Monograph on the Evaluation of the Carcinogenic Risks to Humans, 1994, 61(1).Google Scholar
- 10.B. J. Marshall. Gastroenterology Clinics of North America, 1993, 22(1), 183–98.Google Scholar