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Journal of Structural Chemistry

, Volume 59, Issue 6, pp 1299–1301 | Cite as

Epr Spectroscopy of Adsorbed Spin-Labeled Peptides: Immobilization of Trichogin on the Titanium Oxide

  • R. I. SamoilovaEmail author
  • V. N. Syryamina
  • Yu. D. Tsvetkov
  • S. A. Dzuba
  • C. Toniolo
  • F. Formaggio
Article

Abstract

The TOAC-spin-labeled peptide Trichogin GA IV adsorbed on the TiO2 surface is studied. It is shown that the continuous wave (CW) electron paramagnetic resonance (EPR) spectrum does not depend on temperature in a wide range of 77–300 K. A pulsed EPR method of electron spin echo (ESE) utilizing a two-pulse sequence (π/2-τ-π) is used to study temperature dependence of the phase relaxation time, TF. The TF values are found to change from 750 ns to 100 ns in the interval of 77–300 K. The pulsed electronelectron double resonance (PELDOR) measurements utilizing the pulse sequence((π/2)A,-T-πB,-(τ-T)-πA) show that the space distribution of spin labels on the surface remains uniform irrespective of the temperature, and provide the fractal dimension of the surface of 2.7±0.1. The obtained results testify that EPR pulse experiments can be used to study adsorbed spin-labeled molecules at room temperatures, i.e. not only at cryogenic temperatures.

Keywords

ТОАС spin-labeled peptide immobilization of titanium oxide EPR spectroscopy 

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • R. I. Samoilova
    • 1
    Email author
  • V. N. Syryamina
    • 1
    • 2
  • Yu. D. Tsvetkov
    • 1
  • S. A. Dzuba
    • 1
    • 2
  • C. Toniolo
    • 3
  • F. Formaggio
    • 3
    • 4
  1. 1.Voevodsky Institute of Chemical Kinetics and CombustionNovosibirskRussia
  2. 2.Department of PhysicsNovosibirsk State UniversityNovosibirskRussia
  3. 3.Department of Organic ChemistryUniversity of PadovaPadovaItaly
  4. 4.Department of Chemistry and BiochemistryUniversity of PadovaPadovaItaly

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