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Doklady Chemistry

, Volume 483, Issue 1, pp 268–271 | Cite as

Synthesis and Testing of Abscisic Acid with Predominant Replacement of Protium Atoms by Tritium in the Cyclohexene Moiety

  • V. P. ShevchenkoEmail author
  • I. Yu. Nagaev
  • A. I. Shaposhnikov
  • K. V. Shevchenko
  • A. A. Belimov
  • S. N. Batasheva
  • N. E. Gogoleva
  • Yu. V. Gogolev
  • N. F. Myasoedov
CHEMISTRY
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Abstract

A procedure for tritiation of predominantly the cyclohexene moiety of abscisic acid was developed. Tritium was introduced by isotope exchange reaction with 100% tritiated water at 220°C in the presence of diisopropylethylamine. The yield of abscisic acid was 50%, and the specific activity was 30.5 Ci/mmol. The labeled product was tested. It was shown that tritiated abscisic acid synthesized by the proposed method did not differ from the unlabeled precursor and could be used for biological assays.

Notes

ACKNOWLEDGMENTS

This work was partially supported by the Russian Science Foundation (project no. 17–14–01363) and is a part of government order in an R&D topic (State registration no. 01201355494) and the Program for Competitive Growth of the Kazan Federal University.

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • V. P. Shevchenko
    • 1
    Email author
  • I. Yu. Nagaev
    • 1
  • A. I. Shaposhnikov
    • 2
  • K. V. Shevchenko
    • 1
  • A. A. Belimov
    • 2
  • S. N. Batasheva
    • 3
  • N. E. Gogoleva
    • 3
    • 4
  • Yu. V. Gogolev
    • 3
    • 4
  • N. F. Myasoedov
    • 1
  1. 1.Institute of Molecular Genetics, Russian Academy of SciencesMoscowRussia
  2. 2.All-Russian Research Institute for Agricultural MicrobiologySt. PetersburgRussia
  3. 3.Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of SciencesKazanRussia
  4. 4.Kazan (Volga Region) Federal UniversityKazan,Russia

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