Synthesis of Tritiated Glutamic Acid and Pyroglutamate
- 14 Downloads
It was shown that introduction of tritium directly into glutamic acid does not afford a highly labeled product (at 160°C, a specimen with a specific radioactivity of 3–5 Ci/mmol was formed; at 180°C, the specific radioactivity was 26 Ci/mmol; and at 200°C, the compound strongly degraded). Heating a mixture of 5% Pd/Al2O3 with alumina-supported sodium glutamate at 220°C for 7.5 min gave mainly [3H]pyroglutamate. After isolation and purification of [3H]pyroglutamate, its solution in 1 N HCl was heated at 105°C for 20 h. This yielded [3H]glutamic acid with a specific radioactivity of 40 Ci/mmol.
This work was partially supported by the Russian Science Foundation (project no. 16–14–00077) and Programs of the Presidium of the RAS “Fundamental Research for the Development of Biomedical Technologies” and “Molecular and Cell Biology and Post-Genomic Technologies.”
- 1.Shevchenko, V.P., Nagaev, I.Yu., and Myasoedov, N.F., Mechennye tritiem lipofil’nye soedineniya (Tritium-Labeled Lipophilic Compounds), Moscow: Nauka, 2003.Google Scholar
- 2.Toth, G., Mallareddy, J.R., Toth, F., Lipkowski, A.W., and Tourwe, D., ARKIVOC, vol. 2012, pp. 163–174.Google Scholar
- 3.Rees, A.T., J. Label. Comp. Radiopharm., 2004, vol. 47, no. 5, pp. 318–320.Google Scholar
- 7.Shevchenko, V.P., Radilov, A.S., Nagaev, I.Yu., Shevchenko, K.V., Rembovskii, V.R., and Myasoedov, N.F., Radiokhimiya, 2015, vol. 57, no. 5, pp. 463–465.Google Scholar