Doklady Biological Sciences

, Volume 488, Issue 1, pp 156–159 | Cite as

Immunohistochemical and Morphological Study of Periodontal Tissues in Predicting the Results of Dental Implantation in Patients with Chronic Periodontitis

  • A. A. Kulakov
  • E. A. Kogan
  • T. V. Brailovskaya
  • A. P. VedyaevaEmail author
  • N. V. Zharkov


Morphological and immunohistochemical examination was made on 24 gum biopsies obtained from 35- to 60-year-old patients with diagnosis of partial secondary adentia, chronic generalized moderate to severe periodontitis (19 patients), as well as on the biopsy samples from five patients without pathological periodontal changes who underwent dental implantation. Serial paraffin sections were treated with antibodies against Ki-67, VEGF, and SMA. In patients with severe chronic periodontitis, a high proliferative activity of epithelium indicative of hyperplastic changes was observed, as well as a reduced number of the SMA-positive cells and actual absence of the SMA-positive cell couplings associated with the “growth zones” in tissues, which testifies indirectly to a lower tissue regenerative capacity. Hence, before dental implantation, additional anti-inflammatory and pro-regenerative treatment is required.



The research was supported by a grant from the First Sechenov Moscow State Medical University, Ministry of Health, Russian Federation (Moscow, 2019).


Conflict of interests. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • A. A. Kulakov
    • 1
    • 2
  • E. A. Kogan
    • 1
  • T. V. Brailovskaya
    • 1
    • 2
  • A. P. Vedyaeva
    • 1
    • 2
    Email author
  • N. V. Zharkov
    • 1
  1. 1.First Sechenov Moscow State Medical University, Ministry of HealthMoscowRussia
  2. 2.Central Research Institute of Dentistry and Maxillofacial Surgery, Ministry of Health of the Russian FederationMoscowRussia

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