Features of Ca2+ signaling in proliferating and in differentiating murine myoblasts
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The state of the Ca2+ signaling system has been assessed in proliferating and in differentiating C2C12 myoblasts. Proliferating myoblasts exhibit no features of a functional system: the intracellular ATP-controlled Ca2+ store is low (perhaps only mitochondrial) and no Ca2+ entry from the medium is registered upon its exhaustion, there is no cytosolic response to KCl-induced depolarization. The Ca2+ signaling system starts to form at the early stages of differentiation (within 10 h after transfer of cells to the differentiation medium). This is seen as appearance of capacitive and voltage-dependent Ca2+ entry and its accumulation in the endoplasmic reticulum. A small contribution to the ATP-evoked rise in cytosolic Ca2+ is also made by mitochondria.
Keywordsmyoblast proliferation differentiation Ca2+ entry ATP Ca2+ signaling
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- 1.L. P. Dolgacheva, M. V. Konakov, T. A. Agafonova, et al., Tsitologiya 49(12), 1032 (2007).Google Scholar
- 2.A. S. Baumuratov, B. B. Abzhzlelov, L. I. Kramarova, et al., Byul. Eksper. Biol. Med. 138(7), 59 (2004).Google Scholar
- 4.F. Naro, V. De Arcangelis, D. Coletti, et al., Am. J. Physiol. Cell Physiol. 284(4), 969 (2003).Google Scholar
- 5.O. V. Balan, E. A. Vorotelyak, T. S. Smirnova, et al., Izv. RAN Ser. Biol. 2, 151 (2008).Google Scholar
- 7.S. Arkle, I. M. Wormstone, Z. A. Bascal, et al., Exp. Physiol. 79(6), 975 (1994).Google Scholar
- 8.A. S. Gukovskaya and V. P. Zinchenko, Phys. Chem. Biol. 10, 1 (1990).Google Scholar
- 10.P. G. Suh, J. I. Park, L. Manzoli, et al., BMB Rep. 41(6), 415 (2008).Google Scholar