, Volume 55, Issue 4, pp 573–576 | Cite as

Features of Ca2+ signaling in proliferating and in differentiating murine myoblasts

  • A. M. KrasnyiEmail author
  • N. D. Ozernyuk
Cell Biophysics


The state of the Ca2+ signaling system has been assessed in proliferating and in differentiating C2C12 myoblasts. Proliferating myoblasts exhibit no features of a functional system: the intracellular ATP-controlled Ca2+ store is low (perhaps only mitochondrial) and no Ca2+ entry from the medium is registered upon its exhaustion, there is no cytosolic response to KCl-induced depolarization. The Ca2+ signaling system starts to form at the early stages of differentiation (within 10 h after transfer of cells to the differentiation medium). This is seen as appearance of capacitive and voltage-dependent Ca2+ entry and its accumulation in the endoplasmic reticulum. A small contribution to the ATP-evoked rise in cytosolic Ca2+ is also made by mitochondria.


myoblast proliferation differentiation Ca2+ entry ATP Ca2+ signaling 


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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Koltsov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia

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