Biophysics

, Volume 54, Issue 5, pp 584–589

Correction of spectra for studying dye aggregates by resonance light scattering

  • A. M. Tikhomirov
  • T. A. Shmigol
  • E. A. Kozhinova
  • A. A. Kyagova
  • L. N. Bezdetnaya
  • A. Ya. Potapenko
Molecular Biophysics

DOI: 10.1134/S0006350909050054

Cite this article as:
Tikhomirov, A.M., Shmigol, T.A., Kozhinova, E.A. et al. BIOPHYSICS (2009) 54: 584. doi:10.1134/S0006350909050054

Abstract

Resonance light scattering (RLS), a phenomenon of abrupt enhancement of Rayleigh light scattering in close proximity to an absorption band, is easily detectable in solutions of strongly absorbing chromophores, which form large aggregates with strong π-electronic coupling among the chromophores. RLS spectra need to be corrected for the sensitivity of the spectrofluorimeter as well as for the effects of internal light filter. A method for correcting the measured RLS is described. It was shown by the method that addition of KCl induces formation of extended supramolecular aggregates (probably of H-type) of the anionic dye merocyanine 540 in water. The RLS spectra of a photosensitizer m-tetra(hydroxyphenyl)chlorin (Foscan®) indicate formation of J-aggregates of this dye in aqueous medium.

Key words

aggregation dyes merocyanine 540 mTHPC m-tetra(hydroxyphenyl)chlorine resonance light scattering 

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. M. Tikhomirov
    • 1
  • T. A. Shmigol
    • 1
  • E. A. Kozhinova
    • 1
  • A. A. Kyagova
    • 1
  • L. N. Bezdetnaya
    • 2
  • A. Ya. Potapenko
    • 1
  1. 1.Russian State Medical UniversityMoscowRussia
  2. 2.CRAN, CNRS, Nancy - UniversityVandoeuvre-les-NancyFrance

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