Biochemistry (Moscow)

, Volume 84, Issue 5, pp 570–574 | Cite as

Properties of Rhodobacter sphaeroides Reaction Centers with the Ile→Tyr Substitution at Positions L177 and M206

  • T. Yu. FufinaEmail author
  • G. K. Selikhanov
  • I. I. Proskuryakov
  • V. A. Shuvalov
  • L. G. Vasilieva


Studying pigment-protein interactions in the photosynthetic reaction centers (RCs) is important for the understanding of detailed mechanisms of the photochemical process. This paper describes spectral and photochemical characteristics, pigment composition, and stability of the Rhodobacter sphaeroides RCs with the I(L177)Y and I(M206)Y amino acid substitutions. The obtained data are compared with the properties of I(L177)H, I(L177)D, and I(M206)H RCs reported previously. It is shown that the I(L177)Y and I(M206)Y mutations cause a similar shift of the QYP band in the absorption spectra of the mutant RCs and do not affect the distribution of the electron spin density within the photo-oxidized P+ dimer. The differences in the position and amplitude of the QYB band in the I(L177)Y and I(M206)Y RCs were determined. The results indicate the possibility of new pigment-protein interactions in the vicinity of monomeric bacteriochlorophylls in the A and B chains, which might be of interest for future research.


Rhodobacter sphaeroides photosynthetic reaction center pigment-protein interactions purple bacteria site-directed mutagenesis 


BA and BB

monomeric bacteriochlorophylls






bacteriochlorophyll special pair


oxidized primary electron donor

PA and PB

bacteriochlorophylls from the special pair


reaction center


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Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  • T. Yu. Fufina
    • 1
    Email author
  • G. K. Selikhanov
    • 2
  • I. I. Proskuryakov
    • 1
  • V. A. Shuvalov
    • 1
  • L. G. Vasilieva
    • 1
  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia
  2. 2.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

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