Biochemistry (Moscow)

, Volume 84, Issue 1, pp 1–10 | Cite as

Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells

  • E. G. OrlovaEmail author
  • S. V. Shirshev
  • O. A. Loginova


Molecular mechanisms of the immunomodulatory effects of leptin and ghrelin in concentrations typical for preg–nancy on the maturation and functional activity of dendritic cells (DCs) generated from the peripheral blood monocytes of women are investigated. The presence of leptin during DC maturation did not affect the levels of CD83+CD1c+, CD86+CD1c+, and HLA–DR+CD1c+ DCs, but increased the amount and the activity of the enzyme indoleamine 2,3–dioxygenase (IDO). Cell culturing in the presence of ghrelin or combination of leptin and ghrelin reduced the percentage of CD86+CD1c+ DCs but did not affect the levels of CD83+CD1c+ and HLA–DR+CD1c+ DCs. In addition, ghrelin reduced the number of IDO molecules without affecting its activity. Simultaneous presence of leptin and ghrelin increased induced IDO activity without affecting the amount of the enzyme in DCs. The effects of leptin and ghrelin on the investi–gated functions of DCs in some cases correlated with high levels of cAMP. New mechanisms for leptin and ghrelin regula–tion of tolerogenic functions of DCs in pregnancy are proposed.


leptin ghrelin pregnancy dendritic cells IDO cAMP 



adenylate cyclase


AMP-activated protein kinase




3′,5′-cyclic adenosine monophosphate


clusters of differentiation, molecule expressed on the surface of immune system cells


cAMP response element-binding (CRE)




dendritic cell


exchange protein directly activated by cAMP


extracellular signal-regulated kinase


receptor of ghrelin


human leukocyte antigen DR


indoleamine 2,3-dioxygenase




inositol 1,4,5-triphosphate


immunoreceptor tyrosine-based inhibito-ry motifs 2


Janus kinase


c-Jun N-terminal kinase


receptor of leptin




mito-gen-activated protein kinase


myeloid differentiation primary response 88


nuclear factor kappa-light-chain-enhancer of activated B cells


p38 mitogen-activated protein kinase


phosphodiesterase 3B


phospho-inositide 3-kinase


phosphatidylinositol 4,5-bisphosphate


protein kinase A


protein kinase B


pro-tein kinase C


phospholipase C


suppressors of cytokine signaling 3


signal transducer and activator of tran-scription-3


Toll-like receptor-4


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Copyright information

© Pleiades Publishing, Inc. 2019

Authors and Affiliations

  • E. G. Orlova
    • 1
    Email author
  • S. V. Shirshev
    • 1
  • O. A. Loginova
    • 1
  1. 1.Perm Federal Research Center, Institute of Ecology and Genetics of MicroorganismsUral Branch of the Russian Academy of SciencesPermRussia

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