Biochemistry (Moscow)

, Volume 83, Issue 12–13, pp 1552–1562 | Cite as

Application of the CRISPR/Cas System for Generation of Pathogen-Resistant Plants

  • S. S. Makarova
  • A. V. Khromov
  • N. A. Spechenkova
  • M. E. Taliansky
  • N. O. KalininaEmail author


The use of the CRISPR/Cas9 prokaryotic adaptive immune system has led to a breakthrough in targeted genome editing in eukaryotes. The CRISPR/Cas technology allows to generate organisms with desirable characteristics by introducing deletions/insertions into selected genome loci resulting in the knockout or modification of target genes. This review focuses on the current state of the CRISPR/Cas use for the generation of plants resistant to viruses, bacteria, and parasitic fungi. Resistance to DNA–and RNA–containing viruses is usually provided by expression in transgenic plants of the Cas endonuclease gene and short guide RNAs (sgRNAs) targeting certain sites in the viral or the host plant genomes to ensure either direct cleavage of the viral genome or modification of the plant host genome in order to decrease the efficiency of virus replication. Editing of plant genes involved in the defense response to pathogens increases plants resistance to bacteria and pathogenic fungi. The review explores strategies and prospects of the development of pathogen–resistant plants with a focus on the generation of non–transgenic (non–genetically modified) organisms, in particular, by using plasmid (DNA)–free systems for delivery of the Cas/sgRNA editing complex into plant cells.


plants CRISPR/Cas system genome editing plant viruses bacteria fungi resistance to pathogen infection 



Bean yellow dwarf virus


Beet severe curly top virus


CRISPR–associated endonuclease 9


Cotton leaf curl Kokhran virus


Cucumber mosaic virus


clustered regularly interspaced short palindromic repeats


Cucumber vein yellowing virus


genetically modified organism


intergenic region


Merremia mosaic virus


protospacer–associated motif


Papaya ring spot mosaic virus


short guide RNA


Tomato golden mosaic virus


Turnip mosaic virus


Tomato yellow leaf curl virus


Zucchini yellow mosaic virus


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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • S. S. Makarova
    • 1
    • 2
  • A. V. Khromov
    • 1
    • 2
  • N. A. Spechenkova
    • 3
  • M. E. Taliansky
    • 1
    • 3
  • N. O. Kalinina
    • 1
    • 2
    • 3
    Email author
  1. 1.Doka-Gene Technology Ltd.Rogachevo, Moscow RegionRussia
  2. 2.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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