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Biochemistry (Moscow)

, Volume 83, Issue 12–13, pp 1534–1542 | Cite as

Potential Markers of Autoimmune Diseases, Alleles rs115662534(T) and rs548231435(C), Disrupt the Binding of Transcription Factors STAT1 and EBF1 to the Regulatory Elements of Human CD40 Gene

  • L. V. Putlyaeva
  • D. E. Demin
  • K. V. Korneev
  • A. S. Kasyanov
  • K. A. Tatosyan
  • I. V. Kulakovskiy
  • D. V. Kuprash
  • A. M. SchwartzEmail author
Article

Abstract

CD40 receptor is expressed on B lymphocytes and other professional antigen–presenting cells. The binding of CD40 to its ligand CD154 on the surface of T helper cells plays an important role in the activation of B lymphocytes required for production of antibodies, in particular, against autoantigens. Association of several single nucleotide polymorphisms (SNPs) located in the non–coding areas of human CD40 locus with the elevated risk of autoimmune diseases has been demonstrated. The most studied of these SNPs is rs4810485 located in the first intron of the CD40 gene. Expression of the CD40 gene in B lymphocytes of donors homozygous for the common allelic variant of this polymorphism (G) is higher than in B cells from donors carrying the minor (T) variant. We investigated the enhancer activity of this fragment of the CD40 locus in human B cell lines and showed that it is independent on the rs4810485 alleles. However, the minor allelic variants of the rs4810485–linked SNPs rs548231435 and rs115662534 were associated with a significant decrease in the activity of the CD40 promoter due to the impairments in the binding of EBF1 and STAT1 transcription factors, respectively.

Keywords

autoimmune diseases polymorphism CD40 transcription regulation 

Abbreviations

SNP

single nucleotide polymorphism

TNF

tumor necrosis factor

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Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • L. V. Putlyaeva
    • 1
  • D. E. Demin
    • 1
    • 2
  • K. V. Korneev
    • 1
    • 3
  • A. S. Kasyanov
    • 4
  • K. A. Tatosyan
    • 1
  • I. V. Kulakovskiy
    • 1
    • 4
    • 5
  • D. V. Kuprash
    • 1
    • 2
    • 3
  • A. M. Schwartz
    • 1
    • 2
    Email author
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Department of Molecular and Biological PhysicsMoscow Institute of Physics and TechnologyDolgoprudny, Moscow RegionRussia
  3. 3.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  4. 4.Vavilov Institute of General GeneticsRussian Academy of SciencesMoscowRussia
  5. 5.Institute of Mathematical Problems of Biology, Keldysh Institute of Applied MathematicsRussian Academy of SciencesPushchino, Moscow RegionRussia

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