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Biochemistry (Moscow)

, Volume 83, Issue 11, pp 1411–1421 | Cite as

Aggregation of Influenza A Virus Nuclear Export Protein

  • A. O. Golovko
  • O. N. KorolevaEmail author
  • A. P. Tolstova
  • N. V. Kuz’mina
  • E. V. Dubrovin
  • V. L. Drutsa
Article
  • 43 Downloads

Abstract

Influenza A virus nuclear export protein (NEP) plays an important role in the viral life cycle. Recombinant NEP proteins containing (His)6-tag at either N-or C-terminus were obtained by heterologous expression in Escherichia coli cells and their high propensity for aggregation was demonstrated. Dynamic light scattering technique was used to study the kinetics and properties of NEP aggregation in solutions under different conditions (pH, ionic strength, presence of low-molecular-weight additives and organic solvents). Using atomic force microscopy, the predominance of spherical aggregates in all examined NEP preparations was shown, with some amyloid-like structures being observed in the case of NEP-C protein. A number of structure prediction programs were used to identify aggregation-prone regions in the NEP structure. All-atom molecular dynamics simulations indicate a high rate of NEP molecule aggregation and reveal the regions preferentially involved in the intermolecular contacts that are located at the edges of the rod-like protein molecule. Our results suggest that NEP aggregation is determined by different types of interactions and represents an intrinsic property of the protein that appears to be necessary for its functioning in vivo.

Keywords

influenza A virus nuclear export protein NEP protein aggregation 

Abbreviations

a.a.

amino acid

AFM

atomic force microscopy

CHAPS

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate

DLS

dynamic light scattering

GuHCl

guanidine hydrochloride

NEP

nuclear export protein

NEP-C and NEP-N

recombinant NEP proteins with (His)6-tag at the C-and N-termini, respectively

PdI

polydis-persity index

Rh

hydrodynamic radius

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. O. Golovko
    • 1
  • O. N. Koroleva
    • 2
    Email author
  • A. P. Tolstova
    • 3
  • N. V. Kuz’mina
    • 4
    • 5
  • E. V. Dubrovin
    • 3
  • V. L. Drutsa
    • 6
  1. 1.Department of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia
  2. 2.Department of ChemistryLomonosov Moscow State UniversityMoscowRussia
  3. 3.Department of PhysicsLomonosov Moscow State UniversityMoscowRussia
  4. 4.Department of BiologyLomonosov Moscow State UniversityMoscowRussia
  5. 5.Frumkin Institute of Physical Chemistry and ElectrochemistryRussian Academy of SciencesMoscowRussia
  6. 6.Belozersky Research Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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