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Biochemistry (Moscow)

, Volume 83, Issue 11, pp 1380–1387 | Cite as

Lymphocyte Phosphatase-Associated Phosphoprotein Is a Substrate of Protein Kinase CK2

  • T. D. Tsoy
  • N. A. Kruglova
  • A. V. FilatovEmail author
Article
  • 25 Downloads

Abstract

Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a molecular partner of CD45 phosphatase that plays a key role in the regulation of antigen-specific activation of lymphocytes. The functions of LPAP still remain unknown. We believe that studying LPAP phosphorylation pathways could shed light on its functions. In this work, we studied the phosphorylation of LPAP ectopically expressed in non-lymphoid cells in order to determine the effect of LPAP interaction partners on its phosphorylation. We found that phosphorylation at Ser153 and Ser163 in non-hematopoietic HEK293 cells was conserved, while phosphorylation at Ser99 and Ser172 was almost absent. The pattern of LPAP phosphorylation in K562 erythroid and U937 myeloid cells expressing endogenous CD45 protein was similar to that observed in T and B lymphocytes. We demonstrated for the first time that LPAP is a substrate for protein kinase CK2 that phosphorylates it at Ser153, presumably ensuring LPAP resistance to degradation.

Keywords

LPAP phosphorylation ectopic expression casein kinase 2 human lymphocytes 

Abbreviations

CIP

calf intestinal phosphatase

CK2

casein kinase 2

2D-DIGE

two-dimensional difference gel electrophoresis

DTT

dithiothreitol

IEF

isoelectric focusing

LPAP

lymphocyte phosphatase-associated phosphoprotein

PAGE

polyacrylamide gel electrophoresis

PMA

phorbol 12-myristate 13-acetate

SDS

sodium dodecyl sulfate

TBB

4,5,6,7-tetrabromo-2-azabenzimidazole

WB

Western blotting

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • T. D. Tsoy
    • 1
    • 2
  • N. A. Kruglova
    • 1
    • 2
  • A. V. Filatov
    • 1
    • 2
    Email author
  1. 1.Institute of Immunology National Research CenterFederal Medical-Biological AgencyMoscowRussia
  2. 2.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia

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