Biochemistry (Moscow)

, Volume 83, Issue 9, pp 1040–1045 | Cite as

Genome Editing and the Problem of Tetraploidy in Cell Modeling of the Genetic Form of Parkinsonism

  • V. V. Simonova
  • A. S. Vetchinova
  • E. V. Novosadova
  • L. G. Khaspekov
  • S. N. IllarioshkinEmail author


The prevalent form of familial parkinsonism is caused by mutations in the LRRK2 gene encoding for the mitochondrial protein kinase. In the review, we discuss possible causes of appearance of tetraploid cells in neuronal precursors obtained from induced pluripotent stem cells from patients with the LRRK2-associated form of parkinsonism after genome editing procedure. As LRRK2 protein participates in cell proliferation and maintenance of the nuclear envelope, spindle fibers, and cytoskeleton, mutations in the LRRK2 gene can affect protein functions and lead, via various mechanisms, to the mitotic machinery disintegration and chromosomal aberration. These abnormalities can appear at different stages of fibroblast reprogramming; therefore, editing of the LRRK2 nucleotide sequence should be done during or before the reprogramming stage.


parkinsonism LRRK2 induced pluripotent stem cells neuronal precursors genome editing tetraploidy 



ataxia telangiectasia mutated


induced pluripotent stem cells


Parkinson’s disease


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • V. V. Simonova
    • 1
  • A. S. Vetchinova
    • 1
  • E. V. Novosadova
    • 2
  • L. G. Khaspekov
    • 1
  • S. N. Illarioshkin
    • 1
    Email author
  1. 1.Research Center of NeurologyMoscowRussia
  2. 2.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia

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