Effects of the Linear Fragments of Beta-(1→3)-Glucans on Cytokine Production in vitro
- 53 Downloads
Beta-glucans, homopolysaccharides composed of 3,6-branching β-(1→3)-D-glucan chains, attract great interest as inducers of cytokine synthesis. In this work, we studied the ability of linear fragments of beta-glucan chains to activate cytokine synthesis. Synthetic nona-β-(1→3)-D-glucoside (SO) representing a linear fragment of beta-glucan chain, endotoxin (ED), and natural β-(1→3)-D-glucan (GL) were tested for their role as inducers of cytokines in whole peripheral blood cultures collected from 17 individuals. The concentrations of IL-12p70, IFN-γ, IL-2, IL-10, IL-8, IL-6, IL-4, IL-5, IL-1β, TNF-α, and TNF-β were measured in the supernatants after 2, 24, and 48 h of cell culturing. SO, ED, and GL stim- ulated production of pro-inflammatory IFN-γ, IL-1β, IL-2, IL-6, IL-8, TNF-α and anti-inflammatory IL-10. The high- est levels of biosynthesis after stimulation with SO were registered for IL-6, IL-8, and TNF-α. SO stimulated production of all cytokines (except IFN-γ) to a lesser extent than ED and GL. The IFN-γ/IL-10 (Th1/Th2) ratios after 24 and 48 h of culturing were 3.1 and 7.5 for SO; 0.03 and 0.1 for GL; and 0.06 and 0.2 for ED, respectively. The results indicate that lin- ear fragments of beta-glucans cause a more pronounced shift of immune response towards the pro-inflammatory (Th1) type than beta-glucan itself.
Keywordscytokines inducers beta-glucans linear fragments of beta-glucans synthetic oligoglucosides
Unable to display preview. Download preview PDF.
- 4.Lehne, G., Haneberg, B., Gaustad, P., Johansen, P. W., Preus, H., and Abrahamsen, T. G. (2006) Oral administration of a new soluble branched beta–1,3–D–glucan is well tolerated and can lead to increased salivary concentrations of immunoglobulin A in healthy volunteers, Clin. Exp. Immunol., 143, 65–69.CrossRefPubMedPubMedCentralGoogle Scholar
- 6.Kurbatova, E. A., Vorobyev, D. S., Akhmatov, E. A., Akhmatova, N. K., Egorova, N. B., Tsvetkov, Yu. E., Sukhova, E. V., Yashunskii, D. V., and Nifantyev, N. E. (2014) Protective activity of glycoconjugate on the basis of synthetic hexasaccharide–related to the fragment of the chain of capsule polysaccharide from Streptococcus pneumoniae serotype 14, Byul. Eksp. Biol. Med., 157, 630–633.Google Scholar
- 7.Akhapkina, I. G., Zheltikova–Vostroknutova, T. M., Antropova, A. B., Egorova, O. V., Kalinkina, M. A., Yashunskii, D. V., Tsvetkov, Yu. E., Karelin, A. A., Nifantyev, N. E., and Mikhailova, N. A. (2015) Immunoenzyme test system for quantification of mycopolysaccharides and their derivatives in the dust of the environment, Patent No. 2543323 of 27.01.2015. RU. (Priority of 4.07.2013).Google Scholar
- 8.Akhapkina, I. G., Zheltikova, T. M., Antropova, A. B., Mikhailova, N. A., Yashunskii, D. V., Karelin, A. A., Khatuntseva, E. A., Tsvetkov, Yu. E., and Nifantyev, N. E. (2015) The development of methodological approaches to creation of reagents for immunoenzyme detection of linear β–(1→3)–glucans and their derivatives in the environment, Immunologiya, 36, 276–279.Google Scholar
- 16.Teke, H. U., Cansu, D. U., Yildiz, P., Temiz, G., and Bal, C. (2017) Clinical significance of serum IL–6, TNF–α, Hepcidin, and EPO levels in anaemia of chronic disease and iron deficiency anaemia: the laboratory indicators for anaemia, Biomed. Res., 28, 2704–2710.Google Scholar
- 17.Bonlokke, J. H., Stridh, G., Sigsgaard, T., Kjaergaard, S. K., Lofstedt, H., Andersson, K., Bonefeld–Jorgensen, E. C., Jayatissa, M. N., Bodin, L., Juto, J. E., and Molhave, L. (2006) Upper–airway inflammation in relation to dust spiked with aldehydes or glucan, Scand. J. Work Environ. Health, 32, 374–382.CrossRefPubMedGoogle Scholar
- 19.Sugiyama, A., Hata, S., Susuki, K., Yoshida, E., Nakano, R., Mitra, S., Arashida, R., Asayama, Y., Yabuta, Y., and Takeuchi, T. (2010) Oral administration of paramylon, a β–1,3–D–glucan isolated from Euglena gracilis Z inhibits development of atopic dermatitis–like skin lesions in NC/Nga mice, J. Vet. Med. Sci., 72, 755–763.CrossRefPubMedGoogle Scholar