Biochemistry (Moscow)

, Volume 83, Issue 6, pp 717–726 | Cite as

Rhamnose-Containing Cell Wall Glycopolymers from Rathayibacter toxicus VKM Ac-1600 and “Rathayibacter tanaceti” VKM Ac-2596

  • A. S. Shashkov
  • E. M. Tul’skayaEmail author
  • A. S. Dmitrenok
  • G. M. Streshinskaya
  • N. V. Potekhina
  • S. N. Senchenkova
  • N. F. Piskunkova
  • L. V. Dorofeeva
  • L. I. Evtushenko


Structures of the cell wall glycopolymers from two representatives of the genus Rathayibacter were investigated using chemical, NMR spectroscopy, and optical methods. The R. toxicus VKM Ac-1600 strain contains two neutral glycopolymers–a linear rhamnomannan →2)-α-D-Rhap-(1→3)-α-D-Manp-(1→ and a branched polysaccharide containing in the repeating unit the residues of D-Manp, D-Glcp, and L-Rhap in the ratios of 2: 4: 1, respectively (the structure is presented in the text). The “Rathayibacter tanaceti” VKM Ac-2596 contains a rhamnomannan that is different from the above-described one by localization of glycosidic bonds on the residues of α-Rhap and α-Manp, i.e. →3)-α-D-Rhap (1→2)-α-D-Manp-(1→. The structures of all identified glycopolymers are described for the first time in actinobacteria. The data obtained make it possible to characterize representatives of the studied actinobacteria more fully and can be used to differentiate Rathayibacter species at the phenotype level.


Rathayibacter cell wall rhamnomannan neutral glycopolymers D-rhamnose NMR spectroscopy 


δC and δH

chemical shifts of 13C and 1H atomsrespectively


correlation spectroscopy


heteronuclear multiple-bond correlation


heteronuclear single quantum coherence


spin-spin interaction constant


one-dimensional rotating frame Overhauser effect spectroscopy


two-dimensional rotating frame Overhauser effect spectroscopy


total correlation spectroscopy


sodium salt of 3-(trimethylsilyl)-3,3,2,2-tetradeuteropropionic acid


All-Russian Collection of Microorganisms


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Supplementary material

10541_2018_626_MOESM1_ESM.pdf (96 kb)
A. S. Shashkov, E. M. Tul’skaya, A. S. Dmitrenok, G. M. Streshinskaya, N. V. Potekhina, S. N. Senchenkova, N. F. Piskunkova, L. V. Dorofeeva, and L. I. Evtushenko, Rhamnose-Containing Cell Wall Glycopolymers from Rathayibacter toxicus VKM Ac-1600 and “Rathayibacter tanaceti” VKM Ac-2596 (ISSN 0006-2979, Biochemistry (Moscow), 2018, Vol. 83, No. 6, pp. 717–726)


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • A. S. Shashkov
    • 1
  • E. M. Tul’skaya
    • 2
    Email author
  • A. S. Dmitrenok
    • 1
  • G. M. Streshinskaya
    • 2
  • N. V. Potekhina
    • 2
  • S. N. Senchenkova
    • 1
  • N. F. Piskunkova
    • 2
  • L. V. Dorofeeva
    • 3
  • L. I. Evtushenko
    • 3
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Lomonosov Moscow State UniversityFaculty of BiologyMoscowRussia
  3. 3.All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchinoRussia

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