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Biochemistry (Moscow)

, Volume 83, Issue 5, pp 595–602 | Cite as

Cytochrome P450 1A1 (CYP1A1) Catalyzes Lipid Peroxidation of Oleic Acid-Induced HepG2 Cells

  • B. Huang
  • J. Bao
  • Y.-R. Cao
  • H.-F. Gao
  • Y. JinEmail author
Article
  • 46 Downloads

Abstract

Nonalcoholic fatty liver disease (NAFLD) is a chronic hepatic disease associated with excessive accumulation of lipids in hepatocytes. As the disease progresses, oxidative stress plays a pivotal role in the development of hepatic lipid peroxidation. Cytochrome P450 1A1 (CYP1A1), a subtype of the cytochrome P450 family, has been shown to be a vital modulator in production of reactive oxygen species. However, the exact role of CYP1A1 in NAFLD is still unclear. The aim of this study was to investigate the effects of CYP1A1 on lipid peroxidation in oleic acid (OA)-treated human hepatoma cells (HepG2). We found that the expression of CYP1A1 is elevated in OA-stimulated HepG2 cells. The results of siRNA transfection analysis indicated that CYP1A1-siRNA inhibited the lipid peroxidation in OA-treated HepG2 cells. Additionally, compared with siRNA-transfected and benzo[a]pyrene (BaP)-OA-induced HepG2 cells, overexpression of CYP1A1 by BaP further accelerated the lipid peroxidation in OA-treated HepG2 cells. These observations reveal a regulatory role of CYP1A1 in liver lipid peroxidation and imply CYP1A1 as a potential therapeutic target.

Keywords

nonalcoholic fatty liver disease (NAFLD) CYP1A1 CYP1A1-siRNA oxidative stress lipid peroxidation 

Abbreviations

BaP

benzo[a]pyrene

CYP or CYP450

cytochrome P450 family

CYP1A1

cytochrome P450 1A1

HepG2 cells

human hepatoma cells

HNE

hydroxynonenal

MDA

malondialdehyde

NAFLD

nonalcoholic fatty liver disease

OA

oleic acid

ROS

reactive oxygen species

siRNA

small interfering RNA

SOD

superoxide dismutase

TAG

triacylglyceride

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • B. Huang
    • 1
  • J. Bao
    • 1
  • Y.-R. Cao
    • 1
  • H.-F. Gao
    • 1
  • Y. Jin
    • 1
    Email author
  1. 1.Key Laboratory of Antiinflammatory and Immune Medicines, Ministry of Education, School of PharmacyAnhui Medical UniversityHefeiChina

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