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Biochemistry (Moscow)

, Volume 83, Issue 2, pp 107–118 | Cite as

Influence of a Single Point Mutation in the Constant Domain of the Bence-Jones Protein BIF on Its Aggregation Properties

  • M. A. TimchenkoEmail author
  • A. A. Timchenko
Article
  • 40 Downloads

Abstract

Multiple myeloma nephropathy occurs due to the aggregate formation by monoclonal immunoglobulin light chains (Bence-Jones proteins) in kidneys of patients with multiple myeloma. The mechanism of amyloid deposit formation is still unclear. Earlier, the key role in the fibril formation has been assigned to the variable domains that acquired amyloidogenic properties as a result of somatic mutations. However, fibril formation by the Bence-Jones protein BIF was found to be the function of its constant domain. The substitution of Ser177 by Asn in the constant domain of the BIF protein is most likely an inherited than a somatic mutation. To study the role of this mutation in amyloidogenesis, the recombinant Bence-Jones protein BIF and its mutant with the N177S substitution typical for the known immunoglobulin Cκ allotypes Km1, Km1,2, and Km3 were isolated. The morphology of aggregates formed by the recombinant proteins under conditions similar to those occurring during the protein transport in bloodstream and its filtration into the renal glomerulus, in the distal tubules, and in the proximal renal tubules was analyzed by atomic force microscopy. The nature of the aggregates formed by BIF and its N177S mutant during incubation for 14 days at 37°C strongly differed and depended on both pH and the presence of a reducing agent. BIF formed fibrils at pH 7.2, 6.5, and 10.1, while the N177S mutant formed fibrils only at alkaline pH 10.1. The refolding of both proteins in the presence of 5 mM dithiothreitol resulted in the formation of branched structures.

Keywords

Bence-Jones proteins myeloma aggregation atomic force microscopy 

Abbreviation

AFM

atomic force microscopy

BJ myeloma

Bence-Jones myeloma

BJPs

Bence-Jones proteins (Ig light chains)

CL

constant domain of Ig light chain

MM

multiple myeloma

VL

variable domain of Ig light chain.

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchino, Moscow RegionRussia
  2. 2.Institute of Protein ResearchRussian Academy of SciencesPushchino, Moscow RegionRussia

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