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Biochemistry (Moscow)

, Volume 83, Issue 1, pp 32–36 | Cite as

Modulation of δ-aminolevulinic acid dehydratase activity by the sorbitol-induced osmotic stress in maize leaf segments

  • M. JainEmail author
  • S. Tiwary
  • R. Gadre
Article
  • 54 Downloads

Abstract

Osmotic stress induced with 1 M sorbitol inhibited δ-aminolevulinic acid dehydratase (ALAD) and aminolevulinic acid (ALA) synthesizing activities in etiolated maize leaf segments during greening; the ALAD activity was inhibited to a greater extent than the ALA synthesis. When the leaves were exposed to light, the ALAD activity increased for the first 8 h, followed by a decrease observed at 16 and 24 h in both sorbitol-treated and untreated leaf tissues. The maximum inhibition of the enzyme activity was observed in the leaf segments incubated with sorbitol for 4 to 8 h. Glutamate increased the ALAD activity in the in vitro enzymatic preparations obtained from the sorbitol-treated leaf segments; sorbitol inhibited the ALAD activity in the preparations from both sorbitol-treated and untreated leaves. It was suggested that sorbitol-induced osmotic stress inhibits the enzyme activity by affecting the ALAD induction during greening and regulating the ALAD steady-state level of ALAD in leaf cells. The protective effect of glutamate on ALAD in the preparations from the sorbitol-treated leaves might be due to its stimulatory effect on the enzyme.

Keywords

aminolevulinic acid dehydratase activity osmotic stress sorbitol maize 

Abbreviations

ALA

5-aminolevulinic acid

ALAD

d-aminolevulinic acid dehydratase

GluTR

glutamyl-tRNA reductase

GSAT

glutamate semialdehyde amino transferase

PBG

porphobilinogen

PBGD

porphobilinogen deaminase

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  1. 1.School of BiochemistryDevi Ahilya Vishwavidyalaya, Takshashila CampusIndore (M.P.)India

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