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Biochemistry (Moscow)

, Volume 82, Issue 12, pp 1521–1528 | Cite as

Y-box-binding protein 1 stimulates abasic site cleavage

  • E. E. Alemasova
  • K. N. Naumenko
  • N. A. Moor
  • O. I. LavrikEmail author
Regular Article

Abstract

Apurinic/apyrimidinic (AP) sites are among the most frequent DNA lesions. The first step in the AP site repair involves the magnesium-dependent enzyme AP endonuclease 1 (APE1) that catalyzes hydrolytic cleavage of the DNA phosphodiester bond at the 5′ side of the AP site, thereby generating a single-strand DNA break flanked by the 3′-OH and 5′-deoxyribose phosphate (dRP) groups. Increased APE1 activity in cancer cells might correlate with tumor chemoresistance to DNA-damaging treatment. It has been previously shown that the multifunctional oncoprotein Y-box-binding protein 1 (YB-1) interacts with APE1 and inhibits APE1-catalyzed hydrolysis of AP sites in single-stranded DNAs. In this work, we demonstrated that YB-1 stabilizes the APE1 complex with double-stranded DNAs containing the AP sites and stimulates cleavage of these AP sites at low magnesium concentrations.

Keywords

Y-box-binding protein 1 AP endonuclease 1 base excision repair apurinic/apyrimidinic site 

Abbreviations

APE1

AP endonuclease 1

AP site

apurinic/apyrimidinic site

YB-1

Y-box-binding protein 1

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • E. E. Alemasova
    • 1
  • K. N. Naumenko
    • 1
    • 2
  • N. A. Moor
    • 1
  • O. I. Lavrik
    • 1
    • 2
    Email author
  1. 1.Institute of Chemical Biology and Fundamental MedicineSiberian Branch of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Novosibirsk State UniversityNovosibirskRussia

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