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Biochemistry (Moscow)

, Volume 82, Issue 12, pp 1513–1520 | Cite as

Effects of mitochondrial antioxidant SkQ1 on biochemical and behavioral parameters in a Parkinsonism model in mice

  • V. V. PavshintsevEmail author
  • L. S. Podshivalova
  • O. Y. Frolova
  • M. V. Belopolskaya
  • O. A. Averina
  • E. A. Kushnir
  • N. V. Marmiy
  • M. L. Lovat
Article

Abstract

According to one hypothesis, Parkinson’s disease pathogenesis is largely caused by dopamine catabolism that is catalyzed on mitochondrial membranes by monoamine oxidase. Reactive oxygen species are formed as a byproduct of these reactions, which can lead to mitochondrial damage followed by cell degeneration and death. In this study, we investigated the effects of administration of the mitochondrial antioxidant SkQ1 on biochemical, immunohistochemical, and behavioral parameters in a Parkinson-like condition caused by protoxin MPTP injections in C57BL/6 mice. SkQ1 administration increased dopamine quantity and decreased signs of sensory-motor deficiency as well as destruction of dopaminergic neurons in the substantia nigra and ventral tegmental area in mice with the Parkinson-like condition.

Keywords

Parkinson’s disease MPTP mitochondrial antioxidants SkQ1 sensorimotor deficiency dopaminergic neurons 

Abbreviations

BW

“Beam walking” test

CNS

central nervous system

DAPI

4′,6-diamidino-2-phenylindole

DOPA

3,4-dihydroxyphenylalanine

DOPAC

3,4-dihydroxyphenylacetic acid

EPM

“Elevated plus maze” test

HEB

hematoencephalic barrier

HPLC

high performance liquid chromatography

MAO-B

monoamine oxidase-B

MPDP+

1-methyl-4-phenyl-2,3-dihydropyridine-ion

MPP+

1-methyl-4-phenyl-pyridinium

MPTP

1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

OF

“Open field” test

PD

Parkinson’s disease

Porsolt

“Porsolt forced swim” test

ROS

reactive oxygen species

SD

sensorimotor deficiency

SkQ1 (PDTP)

plastoquinonyl-decyl-triphenylphosphonium

TH

tyrosine hydroxylase (marker of dopaminergic neurons)

TM

“T-maze” test

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • V. V. Pavshintsev
    • 1
    • 2
    Email author
  • L. S. Podshivalova
    • 2
  • O. Y. Frolova
    • 1
  • M. V. Belopolskaya
    • 1
  • O. A. Averina
    • 2
  • E. A. Kushnir
    • 1
  • N. V. Marmiy
    • 1
  • M. L. Lovat
    • 1
    • 2
  1. 1.Institute of MitoengineeringLomonosov Moscow State UniversityMoscowRussia
  2. 2.Lomonosov Moscow State UniversityFaculty of BiologyMoscowRussia

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