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Biochemistry (Moscow)

, Volume 82, Issue 12, pp 1493–1503 | Cite as

Mitochondria-targeted antioxidant SkQ1 (10-(6′-plastoquinonyl)decyltriphenylphosphonium bromide) inhibits mast cell degranulation in vivo and in vitro

  • M. A. Chelombitko
  • O. A. Averina
  • T. V. Vasilyeva
  • O. Yu. Pletiushkina
  • E. N. Popova
  • A. V. Fedorov
  • B. V. Chernyak
  • V. S. Shishkina
  • O. P. Ilinskaya
Article
  • 25 Downloads

Abstract

The therapeutic effect of mitochondria-targeted antioxidant 10-(6′-plastoquinonyl)decyltriphenylphosphonium bromide (SkQ1) in experimental models of acute inflammation and wound repair has been shown earlier. It was suggested that the antiinflammatory activity of SkQ1 is related to its ability to suppress inflammatory activation of the vascular endothelium and neutrophil migration into tissues. Here, we demonstrated that SkQ1 inhibits activation of mast cells (MCs) followed by their degranulation and histamine release in vivo and in vitro. Intraperitoneal injections of SkQ1 in the mouse air-pouch model reduced the number of leukocytes in the air-pouch cavity and significantly decreased the histamine content in it, as well as suppressing MC degranulation in the air-pouch tissue. The direct effect of SkQ1 on MCs was studied in vitro in the rat basophilic leukemia RBL-2H3 cell line. SkQ1 inhibited induced degranulation of RBL-2H3 cells. These results suggest that mitochondrial reactive oxygen species are involved in the activation of MCs. It is known that MCs play a crucial role in regulation of vascular permeability by secreting histamine. Suppression of MC degranulation by SkQ1 might be a significant factor in the antiinflammatory activity of this mitochondria-targeted antioxidant.

Keywords

inflammation mast cell degranulation histamine mitochondria-targeted antioxidant 

Abbreviations

anti-DNP IgE

mouse monoclonal IgE antibodies against dinitrophenol

DNP-BSA

DNP-conjugated bovine serum albumin; MC, mast cell

mtROS

mitochondrial ROS

PMA

phorbol myristate acetate (phorbol ester)

RBL-2H3

rat basophilic leukemia cell line

ROS

reactive oxygen species

SkQ1

10-(6′-plastoquinonyl)decyltriphenylphosphonium bromide

TGF-β1

transforming growth factor β1

TNF

tumor necrosis factor

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • M. A. Chelombitko
    • 1
  • O. A. Averina
    • 2
  • T. V. Vasilyeva
    • 1
  • O. Yu. Pletiushkina
    • 3
  • E. N. Popova
    • 3
  • A. V. Fedorov
    • 1
  • B. V. Chernyak
    • 3
  • V. S. Shishkina
    • 1
  • O. P. Ilinskaya
    • 1
  1. 1.Lomonosov Moscow State UniversityFaculty of BiologyMoscowRussia
  2. 2.Lomonosov Moscow State UniversityInstitute of MitoengineeringMoscowRussia
  3. 3.Lomonosov Moscow State UniversityBelozersky Institute of Physico-Chemical BiologyMoscowRussia

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