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Biochemistry (Moscow)

, Volume 82, Issue 11, pp 1367–1372 | Cite as

Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine and N-docosahexaenoyl dopamine examined in neuronal precursors derived from human pluripotent stem cells

  • E. V. NovosadovaEmail author
  • E. L. Arsenyeva
  • E. S. Manuilova
  • L. G. Khaspekov
  • M. Yu. Bobrov
  • V. V. Bezuglov
  • S. N. Illarioshkin
  • I. A. GrivennikovEmail author
Article

Abstract

Neuroprotective properties of endocannabinoids N-arachidonoyl dopamine (NADA) and N-docosahexaenoyl dopamine (DHDA) were examined in neuronal precursor cells differentiated from human induced pluripotent stem cells and subjected to oxidative stress. Both compounds exerted neuroprotective activity, which was enhanced by elevating the concentration of the endocannabinoids within the 0.1–10 μM range. However, both agents at 10 μM concentration showed a marked toxic effect resulting in death of ~30% of the cells. Finally, antagonists of cannabinoid receptors as well as the receptor of the TRPV1 endovanilloid system did not hamper the neuroprotective effects of these endocannabinoids.

Keywords

endocannabinoids N-arachidonoyl dopamine N-docosahexaenoyl dopamine neuroprotection induced pluripotent stem cells neural precursors oxidative stress 

Abbreviations

CB1(2)

cannabinoid receptor type 1(2)

DHDA

N-docosahexaenoyl dopamine

ECs

endocannabinoids

ECS

endocannabinoid system

iPSCs

induced pluripotent stem cells

MTT (assay)

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (assay)

NADA

N-arachidonoyl dopamine

ROS

reactive oxygen species

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • E. V. Novosadova
    • 1
    Email author
  • E. L. Arsenyeva
    • 1
  • E. S. Manuilova
    • 1
  • L. G. Khaspekov
    • 2
  • M. Yu. Bobrov
    • 3
    • 4
  • V. V. Bezuglov
    • 3
  • S. N. Illarioshkin
    • 2
  • I. A. Grivennikov
    • 1
    Email author
  1. 1.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia
  2. 2.Research Center of NeurologyMoscowRussia
  3. 3.Shemyakin−Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  4. 4.Kulakov Research Center of Obstetrics, Gynecology, and PerinatologyMinistry of Health of the Russian FederationMoscowRussia

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