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Biochemistry (Moscow)

, Volume 82, Issue 8, pp 933–941 | Cite as

Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition

  • Wen Wang
  • Yan Zhao
  • Shujuan Yao
  • Xiujuan Cui
  • Wenying Pan
  • Wenqian Huang
  • Jiangang Gao
  • Taotao Dong
  • Shiqian Zhang
Article

Abstract

Epithelial ovarian cancer (EOC) has the highest mortality among various types of gynecological malignancies. Most patients die of metastasis and recurrence due to cisplatin resistance. Thus, it is urgent to develop novel therapies to cure this disease. CCK-8 assay showed that nigericin exhibited strong cytotoxicity on A2780 and SKOV3 cell lines. Flow cytometry indicated that nigericin could induce cell cycle arrest at G0/G1 phase and promote cell apoptosis. Boyden chamber assay revealed that nigericin could inhibit migration and invasion in a dose-dependent manner by suppressing epithelial–mesenchymal transition (EMT) in EOC cells. These effects were mediated, at least partly, by the Wnt/β-catenin signaling pathway. Our results demonstrated that nigericin could inhibit EMT during cell invasion and metastasis through the canonical Wnt/β-catenin signaling pathway. Nigericin may prove to be a novel therapeutic strategy that is effective in patients with metastatic EOC.

Keywords

epithelial ovarian cancer nigericin cell cycle epithelial–mesenchymal transition 

Abbreviations

EMT

epithelial–mesenchymal transition

EOC

epithelial ovarian cancer

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • Wen Wang
    • 1
    • 2
  • Yan Zhao
    • 1
    • 3
  • Shujuan Yao
    • 2
  • Xiujuan Cui
    • 2
  • Wenying Pan
    • 1
    • 4
  • Wenqian Huang
    • 1
  • Jiangang Gao
    • 5
  • Taotao Dong
    • 1
  • Shiqian Zhang
    • 1
  1. 1.Department of Obstetrics and GynecologyQilu Hospital, Shandong UniversityJinan, ShandongP.R. China
  2. 2.Department of Obstetrics and GynecologyTengzhou Central People’s HospitalTengzhou, ShandongP.R. China
  3. 3.Department of Obstetrics and GynecologyLinyi People’s HospitalLinyi, ShandongP.R. China
  4. 4.Department of Obstetrics and GynecologyBinzhou Medical School Affiliated HospitalBinzhou, ShandongP.R. China
  5. 5.School of Life ScienceShandong UniversityJinan, ShandongP.R. China

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