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Biochemistry (Moscow)

, Volume 82, Issue 8, pp 894–905 | Cite as

Intracellular localization of apoptotic endonuclease EndoG and splice-variants of telomerase catalytic subunit hTERT

  • D. D. ZhdanovEmail author
  • V. S. Pokrovsky
  • E. V. Orlova
  • V. S. Orlova
  • M. V. Pokrovskaya
  • S. S. Aleksandrova
  • N. N. Sokolov
Article
  • 80 Downloads

Abstract

The activity of telomerase catalytic subunit hTERT (human telomerase reverse transcriptase) can be regulated by alternative splicing of its mRNA. The mechanism of hTERT splicing is not understood in detail. Apoptotic endonuclease EndoG is known to participate in this process. In the present work, the intracellular colocalization and mRNA levels of EndoG and hTERT splice-variants in normal and apoptotic cancer cells were studied. We found that the development of apoptosis increased the expression of EndoG and changed the ratio of hTERT splice-variants, which decreased the telomerase activity in the cells. The development of apoptosis was accompanied by changes in the amount of mRNA and in the localization of EndoG and hTERT splice-variants in the cytoplasm, nuclei, and mitochondria of the cells. The suppression of EndoG expression using RNA interference prevented induction of the α+β–splice-variant of hTERT and inhibition of the telomerase activity. A high degree of the intracellular colocalization of EndoG and hTERT was shown. The changes in the expression and localization of EndoG corresponded with changes in the amount and localization of hTERT splice-variants. These data confirm the participation of EndoG in the alternative splicing of mRNA of the telomerase catalytic subunit and in regulation of the telomerase activity.

Keywords

EndoG telomerase hTERT alternative splicing apoptosis 

Abbreviations

AS

alternative splicing

BSA

bovine serum albumin

hTERT

human telomerase reverse transcriptase

siRNA

small interfering RNA

TRAP

telomeric repeat amplification protocol

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • D. D. Zhdanov
    • 1
    • 2
    Email author
  • V. S. Pokrovsky
    • 1
    • 3
  • E. V. Orlova
    • 4
  • V. S. Orlova
    • 2
  • M. V. Pokrovskaya
    • 1
  • S. S. Aleksandrova
    • 1
  • N. N. Sokolov
    • 1
  1. 1.Orekhovich Institute of Biomedical ChemistryMoscowRussia
  2. 2.Ecological FacultyRussian University of People’s FriendshipMoscowRussia
  3. 3.Blokhin Russian Cancer Research CenterMoscowRussia
  4. 4.Institute of Theoretical and Experimental BiophysicsPushchino, Moscow RegionRussia

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