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Biochemistry (Moscow)

, Volume 82, Issue 5, pp 572–578 | Cite as

Minicircle kinetoplast genome of insect trypanosomatid Leptomonas pyrrhocoris

  • E. S. GerasimovEmail author
  • A. A. Gasparyan
  • I. A. Litus
  • M. D. Logacheva
  • A. A. KolesnikovEmail author
Article

Abstract

We present here the structure of a minicircle population based on transcriptome sequencing of Leptomonas pyrrhocoris. We show that minicircle DNA molecules are dimeric. As in dixenous species, the entire molecule of minicircle DNA is transcribed. This is the first minicircle transcriptome of monoxenous trypanosomatid species determined using NGS technology.

Keywords

trypanosomatids kinetoplast minicircle DNA transcriptome assembly 

Abbreviations

CR

conservative region

CSB

conserved sequence block

gRNA

guide RNA

kp

kinetoplast

kpDNA

kinetoplast DNA

kpRNA

kinetoplast RNA

mt

mitochondrial

NGS

next generation sequencing

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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