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Biochemistry (Moscow)

, Volume 82, Issue 4, pp 483–489 | Cite as

Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid

  • A. A. Kasimova
  • M. M. Shneider
  • N. P. Arbatsky
  • A. V. Popova
  • A. S. Shashkov
  • K. A. Miroshnikov
  • Veeraraghavan Balaji
  • Indranil Biswas
  • Yu. A. KnirelEmail author
Article

Abstract

Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1H and 13C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Galp-(1→6)-β-D-Galp-(1→3)-D-GalpNAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Galp-(1→3)-DGalpNAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.

Keywords

Acinetobacter baumannii capsular polysaccharide structure capsule biosynthesis locus pseudaminic acid glycosyltransferase 

Abbreviations

COSY

correlation spectroscopy

CPS

capsular polysaccharide

HMBC

heteronuclear multiple-bond correlation

HSQC

heteronuclear single-quantum coherence

Pse

pseudaminic acid

RHb

(R)-3-hydroxybutanoyl

ROESY

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY

total correlation spectroscopy

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Copyright information

© Pleiades Publishing, Ltd. 2017

Authors and Affiliations

  • A. A. Kasimova
    • 1
    • 2
  • M. M. Shneider
    • 3
  • N. P. Arbatsky
    • 1
  • A. V. Popova
    • 4
    • 5
  • A. S. Shashkov
    • 1
  • K. A. Miroshnikov
    • 3
  • Veeraraghavan Balaji
    • 6
  • Indranil Biswas
    • 7
  • Yu. A. Knirel
    • 1
    Email author
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Higher Chemical College of the Russian Academy of SciencesDmitry Mendeleev University of Chemical Technology of RussiaMoscowRussia
  3. 3.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  4. 4.Moscow Institute of Physics and TechnologyDolgoprudny, Moscow RegionRussia
  5. 5.State Research Center for Applied Microbiology and BiotechnologyObolensk, Moscow RegionRussia
  6. 6.Department of Clinical MicrobiologyChristian Medical College, VelloreTamil NaduIndia
  7. 7.Department of MicrobiologyMolecular Genetics and Immunology, University of Kansas Medical CenterKansas CityUSA

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