Biochemistry (Moscow)

, Volume 81, Issue 13, pp 1754–1770 | Cite as

Immuno-PCR: achievements and perspectives

  • D. Y. RyazantsevEmail author
  • D. V. Voronina
  • S. K. Zavriev


The immuno-PCR (iPCR) method combines advantages of enzyme-linked immunosorbent assay and polymerase chain reaction, which is used in iPCR as a method of “visualization” of antigen–antibody interaction. The use of iPCR provides classical PCR sensitivity to objects traditionally detected by ELISA. This method could be very sensitive and allow for detection of quantities of femtograms/ml order. However, iPCR is still not widely used. The aim of this review is to highlight the special features of the iPCR method and to show the main aspects of its development and application in recent years.


immuno-PCR quantitative PCR antibody antigen DNA–antibody conjugate 



binding antibodies


(biotinylated) detecting antibodies


enzyme-linked immunosorbent assay




polymerase chain reaction


proximity ligation assay


quantitative PCR (realtime PCR)


PCR with reverse transcription


sulfo-N-succinimidyl 4-(maleimidomethyl)cyclo-hexane-1-carboxylate


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • D. Y. Ryazantsev
    • 1
    Email author
  • D. V. Voronina
    • 1
  • S. K. Zavriev
    • 1
  1. 1.Shemyakin–Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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