Study of immunomodulatory effects of extracellular HSP70 in a mouse model of allergic airway inflammation
Immunostimulatory properties of extracellular heat shock proteins 70 kDa (HSP70) became interesting for investigators a long time ago. However, in recent years a series of works showing a significant relation of the immunostimulating effects of recombinant HSP70 to contamination of the protein samples with bacterial endotoxins (lipopolysaccharide, LPS) has been published. The authors showed that intensive elimination of LPS from the protein samples resulted in inversion of immunostimulating effects of HSP70 to immunosuppressive activity of the protein. Nevertheless, at present the conception of immunostimulating, proinflammatory action of extracellular HSP70 is the most common. In this work, we studied immunomodulatory effects of exogenous HSP70 in a mouse model of allergic inflammation of airways. We also analyzed the dynamics of the level of the extracellular pool of HSP70 in the site of inflammation. The results demonstrated a considerable content of extracellular HSP70 in bronchoalveolar lavages with dynamics reflecting the stages of development of the induced inflammation. Oropharyngeal injection of exogenous HSP70 in the acute phase of allergic inflammation of airways resulted in significant suppression of the inflammatory process, which conforms to published data demonstrating an immunosuppressive activity of the extracellular pool of HSP70.
Keywordsheat shock protein 70 kDa HSP70 airway allergic inflammation immunomodulatory effects
antigen presenting cells
bovine serum albumin
enzyme-linked immunosorbent assay
USA Food and Drug Administration
forward small-angle light scattering/side light scattering
heat shock protein 70 kDa
chicken egg albumin
- OVA/OVA mice
mice with ovainduced allergic airway inflammation (ones that got ova both i.p. and o.p.)
phosphate buffered saline
T-helper type 2.
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