Advertisement

Biochemistry (Moscow)

, Volume 81, Issue 10, pp 1111–1117 | Cite as

Interaction between Saccharomyces cerevisiae mitochondrial DNA-binding protein Abf2p and Cce1p resolvase

  • E. O. Samoilova
  • I. A. Krasheninnikov
  • S. A. LevitskiiEmail author
Article

Abstract

Mitochondrial DNA is susceptible to the action of reactive oxygen species generated by the reactions of oxidative phosphorylation. Homologous recombination is one of the mechanisms providing integrity of the mitochondrial genome. Some proteins that take part in this process in budding yeast mitochondria have been identified. These include Abf2p, the major protein of the mt-nucleoid that specifically binds cruciform DNA, and Cce1p – Holliday junction resolvase. Here we show that Abf2p does not significantly affect either binding of Cce1p to branched DNA or rate and specificity of Holliday junction resolution. These data suggest the existence of an alternative homologous recombination pathway in yeast mitochondria.

Key words

mitochondrion homologous recombination Cce1p Abf2p 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Miyakawa, I., Miyamoto, M., Kuroiwa, T., and Sando, N. (2004) DNA content of individual mitochondrial nucleoids varies depending on the culture conditions of the yeast Saccharomyces cerevisiae, Cytologia (Tokyo), 69, 101–107.CrossRefGoogle Scholar
  2. 2.
    Kucej, M., Kucejova, B., Subramanian, R., Chen, X. J., and Butow, R. A. (2008) Mitochondrial nucleoids undergo remodeling in response to metabolic cues, J. Cell Sci., 121, 1861–1868.CrossRefPubMedGoogle Scholar
  3. 3.
    Brown, W. M., George, M., and Wilson, A. C. (1979) Rapid evolution of animal mitochondrial DNA, Proc. Natl. Acad. Sci. USA, 76, 1967–1971.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Chen, X. J. (2013) Mechanism of homologous recombination and implications for aging-related deletions in mitochondrial DNA, Microbiol. Mol. Biol. Rev., 77, 476–496.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    MacAlpine, D. M., Perlman, P. S., and Butow, R. A. (1998) The high mobility group protein Abf2p influences the level of yeast mitochondrial DNA recombination intermediates in vivo, Proc. Natl. Acad. Sci. USA, 95, 6739–6743.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Reeves, R. (2015) High mobility group (HMG) proteins: modulators of chromatin structure and DNA repair in mammalian cells, DNA Repair (Amst.), 36, 122–136.CrossRefGoogle Scholar
  7. 7.
    Megraw, T. L., and Chae, C.-B. (1993) Functional complementarity between the HMGl-like yeast mitochondrial histone HM and the bacterial histone-like protein HU, J. Biol. Chem., 268, 12758–12763.PubMedGoogle Scholar
  8. 8.
    Kurashenko, A. V., Samoilova, E. O., Baleva, M. V., Chicherin, I. V., Petrov, D. Yu., Kamenski, P. A., and Levitskii, S. A. (2016) Two HMG domains of yeast mitochondrial protein Abf2p have different affinity to DNA, Bull. Ross. State Med. Univ., 1, 62–65.Google Scholar
  9. 9.
    Diffley, J. F., and Stillman, B. (1992) DNA binding properties of an HMG1-related protein from yeast mitochondria, J. Biol. Chem., 267, 3368–3374.PubMedGoogle Scholar
  10. 10.
    Sia, R. A., Carrol, S., Kalifa, L., Hochmuth, C., and Sia, E. A. (2009) Loss of the mitochondrial nucleoid protein, Abf2p, destabilizes repetitive DNA in the yeast mitochondrial genome, Genetics, 181, 331–334.PubMedGoogle Scholar
  11. 11.
    Bakkaiova, J., Marini, V., Willcox, S., Nosek, J., Griffith, J. D., Krejci, L., and Tomaska, L. (2016) Yeast mitochondrial HMG proteins: DNA-binding properties of the most evolutionarily divergent component of mitochondrial nucleoids, Biosci. Rep., 36, e00288.CrossRefPubMedCentralGoogle Scholar
  12. 12.
    West, S. C., Parsons, C. A., and Picksley, S. M. (1987) Purification and properties of a nuclease from Saccharomyces cerevisiae that cleaves DNA at cruciform junctions, J. Biol. Chem., 262, 12752–12758.PubMedGoogle Scholar
  13. 13.
    Fogg, J. M., Schofield, M. J., Declais, A. C., and Lilley, D. M. (2000) Yeast resolving enzyme CCE1 makes sequential cleavages in DNA junctions within the lifetime of the complex, Biochemistry, 39, 4082–4089.CrossRefPubMedGoogle Scholar
  14. 14.
    White, M. F., and Lilley, D. M. (1996) The structure-selectivity and sequence-preference of the junction-resolving enzyme CCE1 of Saccharomyces cerevisiae, J. Mol. Biol., 257, 330–341.CrossRefPubMedGoogle Scholar
  15. 15.
    Samoilova, E. O., Krasheninnikov, I. A., Vinogradova, E. N., Kamenski, P. A., and Levitskii, S. A. (2016) Abf2p increases efficiency of DNA uptake by isolated mitochondria, Biochemistry (Moscow), 81, 723–730.CrossRefGoogle Scholar
  16. 16.
    Kamashev, D., and Rouviere-Yaniv, J. (2000) The histonelike protein HU binds specifically to DNA recombination and repair intermediates, EMBO J., 19, 6527–6535.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Zelenaya-Troitskaya, O., Newman, S. M., Okamoto, K., Perlman, P. S., and Butow, R. A. (1998) Functions of the high mobility group protein, Abf2p, in mitochondrial DNA segregation, recombination and copy number in Saccharomyces cerevisiae, Genetics, 148, 1763–1776.PubMedGoogle Scholar
  18. 18.
    Lipinski, K. A., Kaniak-Golik, A., and Golik, P. (2010) Maintenance and expression of the S. cerevisiae mitochondrial genome–from genetics to evolution and systems biology, Biochim. Biophys. Acta, 1797, 1086–1098.CrossRefPubMedGoogle Scholar
  19. 19.
    Newman, S. M., Zelenaya-Troitskaya, O., Perlman, P. S., and Butow, R. A. (1996) Analysis of mitochondrial DNA nucleoids in wild-type and a mutant strain of Saccharomyces cerevisiae that lacks the mitochondrial HMG box protein Abf2p, Nucleic Acids Res., 24, 386–393.CrossRefPubMedPubMedCentralGoogle Scholar
  20. 20.
    Mookerjee, S. A., and Sia, E. A. (2006) Overlapping contributions of Msh1p and putative recombination proteins Cce1p, Din7p, and Mhr1p in large-scale recombination and genome sorting events in the mitochondrial genome of Saccharomyces cerevisiae, Mutat. Res. Mol. Mech. Mutagen., 595, 91–106.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • E. O. Samoilova
    • 1
  • I. A. Krasheninnikov
    • 1
  • S. A. Levitskii
    • 1
    Email author
  1. 1.Faculty of BiologyLomonosov Moscow State University119991Russia

Personalised recommendations