Biochemistry (Moscow)

, Volume 81, Issue 10, pp 1081–1088 | Cite as

Procedure for purification of recombinant preMsk1p from E. coli determines its properties as a factor of tRNA import into yeast mitochondria

  • E. V. Smirnova
  • I. V. Chicherin
  • M. V. Baleva
  • N. S. Entelis
  • I. A. Tarassov
  • P. A. KamenskiEmail author


Mitochondrial genomes of many eukaryotic organisms do not code for the full tRNA set necessary for organellar translation. Missing tRNA species are imported from the cytosol. In particular, one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p. In this work, we show that recombinant preMsk1p purified from E. coli in native conditions, when used in an in vitro tRNA import system, demonstrates some properties different from those shown by the renatured protein purified from E. coli in the denatured state. We also discuss the possible mechanistic reasons for this phenomenon.

Key words

mitochondria tRNA tRNA import into mitochondria preMsk1p Eno2p import mechanisms protein conformation 


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • E. V. Smirnova
    • 1
    • 2
    • 3
  • I. V. Chicherin
    • 1
    • 2
  • M. V. Baleva
    • 1
    • 2
  • N. S. Entelis
    • 2
  • I. A. Tarassov
    • 2
  • P. A. Kamenski
    • 1
    Email author
  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.UMR No. 7156 GMGMStrasbourg UniversityStrasbourgFrance
  3. 3.Institute of Plant Molecular BiologyStrasbourg UniversityStrasbourgFrance

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