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Biochemistry (Moscow)

, Volume 81, Issue 9, pp 1023–1030 | Cite as

Interaction of telomeric retroelement HeT-A transcripts and their protein product gag in early embryogenesis of Drosophila

  • I. A. Olovnikov
  • V. V. Morgunova
  • A. A. Mironova
  • M. Y. Kordyukova
  • E. I. Radion
  • O. M. Olenkina
  • N. V. Akulenko
  • A. I. KalmykovaEmail author
Article

Abstract

The telomere is a nucleoprotein complex at the ends of linear chromosomes that protects them from fusion and degradation. The telomere consists of telomeric DNA, a protective protein complex and telomeric RNA. Biogenesis of telomeric transcripts in development is still far from being understood. Drosophila telomeres are elongated by a transposition of specialized telomeric retrotransposons that encode proteins. Using transgenic constructs encoding tagged telomeric protein, we found that transcripts of Drosophila telomeric element HeT-A bind Gag-HeT-A protein encoded by these transcripts. Maternal HeT-A transcripts and Gag-HeT-A form ribonucleoprotein granules around centrosomes, centers of microtubule organization, during blastoderm formation, upon disruption of telomere silencing during oogenesis. The specific localization of HeT-A RNA is dependent on microtubules since disruption of microtubules caused delocalization of HeT-A transcripts. This transgenic system is a valuable model for the study of telomeric RNA biogenesis.

Keywords

telomere development retrotransposon HeT-A Drosophila telomeric RNA 

Abbreviations

DAPI

4′,6-diamidino-2-phenylindole

FISH

fluorescence in situ hybridization

HA

hemagglutinin

piRNA

Piwi interacting RNA

RIP

RNA-binding protein immunoprecipitation

RNAi

RNA interference

RNP

ribonucleoprotein

TERRA

telomeric repeat-containing RNA

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • I. A. Olovnikov
    • 1
  • V. V. Morgunova
    • 1
  • A. A. Mironova
    • 1
  • M. Y. Kordyukova
    • 1
  • E. I. Radion
    • 1
  • O. M. Olenkina
    • 1
  • N. V. Akulenko
    • 1
  • A. I. Kalmykova
    • 1
    Email author
  1. 1.Institute of Molecular GeneticsRussian Academy of SciencesMoscowRussia

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