Biochemistry (Moscow)

, Volume 81, Issue 3, pp 275–283 | Cite as

Dynamics of antagonistic potency of Rhodobacter capsulatus PG lipopolysaccharide against endotoxin-induced effects

  • D. S. KabanovEmail author
  • D. A. Serov
  • S. V. Zubova
  • S. V. Grachev
  • I. R. Prokhorenko


The dynamics of antagonistic potency of lipopolysaccharide (LPS) isolated from Rhodobacter capsulatus PG on the synthesis of proinflammatory (TNF-α, IL-1β, IL-8, IL-6, IFN-γ) and antiinflammatory (IL-10, IL-1Ra) cytokines induced by highly stimulatory endotoxins from Escherichia coli or Salmonella enterica have been studied. Using human whole blood, we have shown that R. capsulatus PG LPS inhibited most pronouncedly the endotoxin-induced synthesis of TNF-α, IL-1β, IL-8, and IL-6 during the first 6 h after endotoxin challenge. Similarly, the endotoxin-induced release of IFN-γ was abolished by R. capsulatus PG LPS as well (24 h). In contrast to the above-mentioned cytokines, the relatively weak antagonistic activity of R. capsulatus PG LPS against endotoxin-triggered production of IL-6 and IL-8 was revealed. Since R. capsulatus PG LPS displays more potent antagonistic activity against deleterious effects of S. enterica LPS than those of E. coli LPS in the cases of such cytokines as IL-1β (6 and 24 h), IL-6 and IL-8 (4 h), we conclude that the effectiveness of protective action of antagonist is mostly determined by the primary lipid A structure of the employed agonist.

Key words

lipopolysaccharide (endotoxin) antagonist cytokine TNF-α IL-1β IL-6 IL-8 IL-10 IL-1Ra IFN-γ 



cluster of differentiation


interferon γ




LPS-binding protein




membrane-anchored form of CD14


myeloid differentiation factor-2, a secreted glycoprotein


myeloid differentiation primary-response gene 88, adaptor-like protein 88 has a Toll/interleukin-1 receptor (TIR) domain




polymorphonuclear leukocytes


soluble form of CD14 not associated with cell membrane


Toll-like receptor 4


TNF receptor 1


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • D. S. Kabanov
    • 1
    Email author
  • D. A. Serov
    • 1
  • S. V. Zubova
    • 1
  • S. V. Grachev
    • 1
  • I. R. Prokhorenko
    • 1
  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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