Biochemistry (Moscow)

, Volume 81, Issue 3, pp 255–262 | Cite as

Tankyrase activity in organs and tissues of mice

  • A. N. KuimovEmail author
  • A. S. Zhozhikashvili
  • V. N. Manskikh
  • L. V. Platonova
  • T. G. Dyuzheva


Tankyrase, one of the NAD+ ADP-ribosyltransferases, is a target for drugs developed for their anticancer and other pharmacological activities. We designed an assay for estimation of the inhibition or activation of the enzyme in pre-clinical studies. In mice, the highest specific activity of tankyrase was observed in thymus, spleen, pancreas, and bone marrow. In murine liver, tankyrase is active in ontogenesis and during reparative regeneration; however, the basal activity is hardly detectable in normal liver and most of other organs of adult animals. We suggest that tankyrase is a part of the tissue growth and repair machinery, while its age-dependent inhibition, when an organism stops growing, turns on phenoptosis.

Key words

ADP-ribosyltransferase Essentiale N hepatic stimulator substance poly(ADP-ribosyl)ation regeneration 



hepatic stimulator substance


poly(ADP-ribose) polymerase




tankyrase inhibitor


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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • A. N. Kuimov
    • 1
    Email author
  • A. S. Zhozhikashvili
    • 1
  • V. N. Manskikh
    • 2
  • L. V. Platonova
    • 3
  • T. G. Dyuzheva
    • 3
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of MitoengineeringLomonosov Moscow State UniversityMoscowRussia
  3. 3.Sechenov First Moscow State Medical UniversityMoscowRussia

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