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Biochemistry (Moscow)

, Volume 81, Issue 3, pp 187–200 | Cite as

Overview of fusion tags for recombinant proteins

  • E. N. KosobokovaEmail author
  • K. A. Skrypnik
  • V. S. Kosorukov
Review

Abstract

Virtually all recombinant proteins are now prepared using fusion domains also known as “tags”. The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.

Key words

fusion tags chromatography enhancement of protein solubility 

Abbreviations

GST

glutathione S-transferase

hG-CSF

human granulocyte colony-stimulating factor

hGM-CSF

human granulocyte-macrophage colony-stimulating factor

hIFN

human interferon

MBP

maltose-binding protein

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Copyright information

© Pleiades Publishing, Ltd. 2016

Authors and Affiliations

  • E. N. Kosobokova
    • 1
    Email author
  • K. A. Skrypnik
    • 1
  • V. S. Kosorukov
    • 1
  1. 1.Blokhin Russian Cancer Research CenterMoscowRussia

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